Inscuteable Antibody (C-8): sc-390728
- Inscuteable Antibody (C-8) is a mouse monoclonal IgG2a Inscuteable antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 806-831 of Inscuteable of Drosophila melanogaster origin
- Inscuteable Antibody (C-8) is recommended for detection of Inscuteable of d origin by WB, IP, IF and ELISA
- Anti-Inscuteable Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Inscuteable Antibody (C-8) for WB applications. These reagents are now offered in bundles with Inscuteable Antibody (C-8) (see ordering information below).
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Inscuteable Antibody (C-8) is a mouse monoclonal IgG2a antibody that detects Inscuteable protein of Drosophila melanogaster origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Inscuteable antibody (C-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Inscuteable plays a crucial role in asymmetric cell division, particularly in Drosophila neuroblasts, where Inscuteable (C-8) monoclonal antibody is essential for studying proper orientation of mitotic spindles along the cell-polarity axis. This orientation ensures daughter cells receive the correct distribution of cellular components, which ultimately influences cell fate and differentiation. Inscuteable (C-8) antibody helps researchers understand how Inscuteable interacts with Partner of Inscuteable protein, facilitating the localization of basal proteins necessary for spindle orientation. The precise regulation of these processes is critical for normal development and tissue homeostasis, making Inscuteable (C-8) monoclonal antibody valuable for studying cellular dynamics and developmental biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Inscuteable Antibody (C-8) References:
- A functional analysis of inscuteable and its roles during Drosophila asymmetric cell divisions. | Tio, M., et al. 1999. J Cell Sci. 112 (Pt 10): 1541-51. PMID: 10212148
- Lineage, cell polarity and inscuteable function in the peripheral nervous system of the Drosophila embryo. | Orgogozo, V., et al. 2001. Development. 128: 631-43. PMID: 11171389
- The Snail protein family regulates neuroblast expression of inscuteable and string, genes involved in asymmetry and cell division in Drosophila. | Ashraf, SI. and Ip, YT. 2001. Development. 128: 4757-67. PMID: 11731456
- A mammalian Partner of inscuteable binds NuMA and regulates mitotic spindle organization. | Du, Q., et al. 2001. Nat Cell Biol. 3: 1069-75. PMID: 11781568
- Membrane targeting and asymmetric localization of Drosophila partner of inscuteable are discrete steps controlled by distinct regions of the protein. | Yu, F., et al. 2002. Mol Cell Biol. 22: 4230-40. PMID: 12024035
- Cell fate decisions in the Drosophila dorsal vessel depend on the multiadapter protein inscuteable. | Popichenko, D. and Paululat, A. 2004. Genesis. 40: 218-22. PMID: 15593331
- Role of inscuteable in orienting asymmetric cell divisions in Drosophila. | Kraut, R., et al. 1996. Nature. 383: 50-5. PMID: 8779714
- Spindle orientation and asymmetric localization in Drosophila: both inscuteable? | Doe, CQ. 1996. Cell. 86: 695-7. PMID: 8797815
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Inscuteable Antibody (C-8) | sc-390728 | 200 µg/ml | $322.00 | |||
Inscuteable Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-526188 | 200 µg Ab; 10 µg BP | $361.00 | |||
Inscuteable Antibody (C-8): m-IgG2a BP-HRP Bundle | sc-546526 | 200 µg Ab; 10 µg BP | $361.00 | |||
Inscuteable Antibody (C-8) AC | sc-390728 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Inscuteable Antibody (C-8) HRP | sc-390728 HRP | 200 µg/ml | $322.00 | |||
Inscuteable Antibody (C-8) FITC | sc-390728 FITC | 200 µg/ml | $336.00 | |||
Inscuteable Antibody (C-8) PE | sc-390728 PE | 200 µg/ml | $349.00 | |||
Inscuteable Antibody (C-8) Alexa Fluor® 488 | sc-390728 AF488 | 200 µg/ml | $364.00 | |||
Inscuteable Antibody (C-8) Alexa Fluor® 546 | sc-390728 AF546 | 200 µg/ml | $364.00 | |||
Inscuteable Antibody (C-8) Alexa Fluor® 594 | sc-390728 AF594 | 200 µg/ml | $364.00 | |||
Inscuteable Antibody (C-8) Alexa Fluor® 647 | sc-390728 AF647 | 200 µg/ml | $364.00 | |||
Inscuteable Antibody (C-8) Alexa Fluor® 680 | sc-390728 AF680 | 200 µg/ml | $364.00 | |||
Inscuteable Antibody (C-8) Alexa Fluor® 790 | sc-390728 AF790 | 200 µg/ml | $364.00 | |||
Inscuteable (C-8) Neutralizing Peptide | sc-390728 P | 100 µg/0.5 ml | $69.00 |