IL-2Rα Double Nickase Plasmid (h): sc-401262-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-2Rα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-2Rα Double Nickase Plasmid (h) and IL-2Rα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL2RA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-2Rα Antibody (C-9): sc-393326
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-2Rα Double Nickase Plasmid (h) | sc-401262-NIC | 20 µg | $410.00 | |||
IL-2Rα Double Nickase Plasmid (h2) | sc-401262-NIC-2 | 20 µg | $410.00 |
IL2RA encodes the interleukin-2 receptor alpha chain (IL-2Rα, CD25), a high-affinity component of the IL-2 receptor complex that partners with IL2RB and IL2RG to mediate IL-2 sensing. Engagement of IL-2Rα-containing receptors promotes JAK1/JAK3-dependent STAT5 signaling and broader transcriptional programs that regulate T cell proliferation, survival, and differentiation, with particular importance in regulatory T cell maintenance and immune homeostasis. CD25 expression is dynamically regulated during lymphocyte activation and helps tune cytokine responsiveness within the IL-2/STAT axis. Dysregulated IL2RA signaling or expression has been associated with immune-mediated pathologies and altered T cell functional states, making it a common target for mechanistic studies in immunology.
IL-2Rα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL2RA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL2RA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL2RA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL2RA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.