IL-12Rβ1 CRISPR Activation Plasmid (h): sc-404602-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-12Rβ1 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- IL-12Rβ1 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by IL-12Rβ1 CRISPR Activation Plasmid (h) and IL-12Rβ1 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the IL12RB1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-12Rβ1 Antibody (A-10): sc-166776
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-12Rβ1 CRISPR Activation Plasmid (h) | sc-404602-ACT | 20 µg | $397.00 | |||
IL-12Rβ1 CRISPR Activation Plasmid (h2) | sc-404602-ACT-2 | 20 µg | $397.00 |
IL12RB1 encodes the interleukin-12 receptor beta 1 (IL-12Rβ1), a shared receptor subunit for IL-12 and IL-23 signaling that is primarily expressed on activated T cells, NK cells, and antigen-presenting cells. Upon cytokine engagement with partnering receptor chains, IL-12Rβ1 supports JAK-STAT pathway activation, promoting IFN-γ production, Th1 differentiation, and coordination of antimicrobial immune responses. Through its role in IL-12/IL-23–dependent signaling networks, IL-12Rβ1 helps shape inflammation and cellular immunity, making it a key node for studies of cytokine-driven immune polarization. Variation or dysregulation in this axis is commonly examined in the context of immunodeficiency phenotypes, susceptibility to intracellular pathogens, and inflammatory immune disorders relevant to human disease biology.
IL-12Rβ1 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous IL12RB1 expression without altering the underlying DNA sequence.
IL-12Rβ1 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the IL12RB1 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the IL12RB1 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous IL-12Rβ1 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native IL12RB1 locus and enabling the study of IL-12Rβ1-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of IL-12Rβ1 pathway restoration in tumor cells with silenced or reduced IL12RB1 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.