Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-10Rβ Double Nickase Plasmid (h): sc-404666-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-10Rβ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-10Rβ Double Nickase Plasmid (h) and IL-10Rβ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL10RB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-10Rβ Antibody (F-6): sc-271969
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-10Rβ Double Nickase Plasmid (h) | sc-404666-NIC | 20 µg | $410.00 | |||
IL-10Rβ Double Nickase Plasmid (h2) | sc-404666-NIC-2 | 20 µg | $410.00 |
IL10RB encodes interleukin-10 receptor subunit beta (IL-10Rβ), an essential accessory chain shared by multiple class II cytokine receptors, including those for IL-10, IL-22, IL-26, and type III interferons (IFN-λ). Upon ligand engagement, IL-10Rβ partners with the corresponding ligand-specific alpha subunit to support JAK1/TYK2 activation and downstream STAT signaling, shaping transcriptional programs that regulate inflammation, epithelial barrier function, and antiviral responses. This receptor subunit is therefore central to cytokine-driven immune homeostasis at mucosal surfaces and in myeloid and lymphoid compartments. Perturbation of IL10RB-dependent signaling is frequently studied in the context of dysregulated cytokine responses relevant to infection biology, inflammatory pathology, and immune-mediated mechanisms.
IL-10Rβ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL10RB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL10RB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL10RB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL10RB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.