Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IGFBP3 Double Nickase Plasmid (h): sc-400682-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGFBP3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IGFBP3 Double Nickase Plasmid (h) and IGFBP3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IGFBP3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IGFBP3 Antibody (E-9): sc-374365
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGFBP3 Double Nickase Plasmid (h) | sc-400682-NIC | 20 µg | $410.00 | |||
IGFBP3 Double Nickase Plasmid (h2) | sc-400682-NIC-2 | 20 µg | $410.00 |
Insulin-like growth factor binding protein 3 (IGFBP3) is a major secreted carrier of IGF-I and IGF-II in human plasma that modulates ligand bioavailability and signaling through IGF1R, thereby influencing PI3K–AKT and MAPK pathway activity. Beyond IGF sequestration, IGFBP3 can engage IGF-independent processes including cell-cycle regulation, apoptosis, senescence, and stress responses via interactions with cell-surface and nuclear partners. Altered IGFBP3 expression or proteolytic processing has been linked to dysregulated growth control and inflammatory microenvironments, making it a frequent target in oncology, metabolism, and tissue remodeling research. Its context-dependent roles support studies of endocrine/paracrine signaling, extracellular matrix crosstalk, and transcriptional programs governing proliferation and survival.
IGFBP3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IGFBP3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IGFBP3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IGFBP3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IGFBP3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.