All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
Id Antibodies for analysis of cellular responses to Id CRISPR Products
For further details describing the CRISPR System
CRISPR/Cas9 KO plasmids, Double Nickase Plasmids and CRISPR/dCas9 Activation plasmids are considered a "Licensed Product" and are to be used in accordance with the Limited License. Click here for details
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Id4 CRISPR Activation Plasmid (h) 는 세개의 plasmid가 1:1:1 의 비례로 포함되여있으며 각각 transactivation domain인 VP64에 융합시킨 deactivated Cas9 (dCas9) 뉴클리아제 (D10A and N863A) 와 blasticidin resistance gene을 인코딩; MS2-p65-HSF1융합프로틴과 hygromycin resistance gene을 인코딩; 타깃특정 20 nt guide RNA와 puromycin resistance gene을 인코딩합니다.
The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
Id4 CRISPR Activation Plasmid (h2) 의 The sgRNA (MS2) plasmid 가 인코딩한 타깃특정 20 nt guide RNA는Id4 CRISPR Activation Plasmid (h)의 The sgRNA (MS2) plasmid 가 인코딩한것과 다릅니다.
Id4 Lentiviral Activation Particles (h) 은 아래의 SAM Activation elements를 포함하고 있습니다: transactivation domain VP64과 융합된 deactivated Cas9 (dCas9) 뉴클리아제 (D10A and N863A),MS2-p65-HSF1융합 프로틴과 타깃특정 20 nt. guide RNA. 또한 각각 blasticidin, hygromycin 과 puromycin resistance genes를 포함하고있습니다.
SAM complex는 transduction후 transcriptional start site상류의 약 200-250 nt의 특정위치에 바인딩하여 고효율gene activation에 필요한 transcription factor을 증가시킵니다.
Id4 Lentiviral Activation Particles (h2) 이 인코딩한 타깃 특정 20 nt guide RNA는 Id4 Lentiviral Activation Particles (h)이 인코딩한것과 다릅니다.