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HSP90 항체 (4F10): sc-69703

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데이터 시트
  • HSP90 항체 4F10 는 마우스 monoclonal IgG2b HSP90 항체, 157간행물에 인용, 이며 50 µg/0.5 ml으로 제공합니다.
  • human HSP 90의 아미노산 잔기 에서의 에피토프 매핑
  • WB, IP 와ELISA으로 human origin의 HSP 90을 검출할것을 권장합니다.
  • See HSP90 alpha/beta (F-8): sc-13119 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • HSP90 (4F10): sc-69703무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 2b BP-HRP">m-IgG2b BP-HRP 는 HSP90 항체 (4F10) WB 애플리케이션용입니다. 이 시약은 이제 HSP90 항체 (4F10)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

빠른 링크

더보기

HSP 90 항체(4F10)는 인간 유래의 HSP 90 단백질을 WB, IP 및 ELISA로 검출하는 IgG2b 마우스 단일 클론 HSP 90 항체(HSP90 항체, HSP90AA1 항체 또는 HSP90AB1 항체로도 지정됨)입니다. HSP 90 항체(4F10)는 비접합 항-HSP 90 항체로 사용할 수 있습니다. 열충격 반응은 초파리 침샘 세포에서 처음 설명되었으며, 형태학적으로 몇 가지 단백질의 신규 합성을 포함하는 다염색체 퍼핑 패턴의 변화로 구성됩니다. 이후 박테리아 닭과 포유류 세포에서 유사한 열충격 단백질이 발견되었고, 이후 다른 생물체에서도 연구가 진행되었습니다. HSP 90, HSP 70, HSP 20-30 및 유비퀴틴을 포함한 일련의 단백질은 온도 충격, 화학 물질 및 기타 환경 스트레스와 같은 충격에 의해 유도됩니다. HSP 90과 다른 HSP의 주요 기능은 분자 샤프론 역할을 하는 것입니다. HSP 90은 글루코코르티코이드 수용체(GR)와 복합체를 형성하여 비리간드 결합 수용체를 전사적으로 비활성화합니다. HSP 90은 HSP 56 또는 사이클로필린 D로 구성된 헤테로복합체로 GR과 결합하여 아포수용체 복합체를 형성합니다. HSP 90은 또한 p60/STI1 및 p23과 같은 다른 단백질과 이합체 형태로 존재하여 에스트로겐 및 안드로겐 수용체와 아포레수용체 복합체를 형성합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

HSP90 항체 (4F10) 참고문헌:

  1. PKC 엡실론은 열충격 반응에서 hsp90 베타 유전자의 독특한 조절인자입니다.  |  Wu, JM., et al. 2003. J Biol Chem. 278: 51143-9. PMID: 14532285
  2. HSP90과 암의 샤프롱.  |  Whitesell, L. and Lindquist, SL. 2005. Nat Rev Cancer. 5: 761-72. PMID: 16175177
  3. Hsp90은 다양한 곰팡이에서 약물 내성과 같은 새로운 형질의 빠른 진화를 촉진합니다.  |  Cowen, LE. and Lindquist, S. 2005. Science. 309: 2185-9. PMID: 16195452
  4. 가역적 아세틸화를 통해 분자 샤프론 Hsp90의 기능을 조절합니다.  |  Aoyagi, S. and Archer, TK. 2005. Trends Cell Biol. 15: 565-7. PMID: 16199163
  5. 인간 게놈의 HSP90 유전자군: 그 분화와 진화에 대한 인사이트.  |  Chen, B., et al. 2005. Genomics. 86: 627-37. PMID: 16269234
  6. Hsp90: 단백질 폴딩과 유전자 조절을 위한 샤프론.  |  Zhao, R. and Houry, WA. 2005. Biochem Cell Biol. 83: 703-10. PMID: 16333321
  7. Hsp90은 센다이 바이러스에 감염된 세포에서 인터페론 조절 인자 3의 활성화와 TBK-1 안정화를 조절합니다.  |  Yang, K., et al. 2006. Mol Biol Cell. 17: 1461-71. PMID: 16394098
  8. 샤프론 Hsp70에서 Hsp90으로의 기질 전달.  |  Wegele, H., et al. 2006. J Mol Biol. 356: 802-11. PMID: 16403523
  9. Hsp90의 C-말단 도메인에서 노보비오신에 의한 샤페론 기능 및 코카페론 상호 작용의 조절: 쿠마린 항생제가 Hsp90 이합체화를 방해한다는 증거.  |  Allan, RK., et al. 2006. J Biol Chem. 281: 7161-71. PMID: 16421106

주문정보

제품명카탈로그 번호 단위가격수량관심품목

HSP90 항체 (4F10)

sc-69703
50 µg/0.5 ml
$316.00

HSP90 (4F10): m-IgG2b BP-HRP 번들

sc-548446
50 µg Ab; 10 µg BP
$354.00

Does the antibody bind to the n-terminal ATP binding site of HSP90?

Asked by: Anonymous
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2020-07-26

Hello, I was wondering what are two bands presented on your Western image. Thanks

Asked by: Sanja
Thank you for your question. The Western blot data you are referring to is in a human HSP 90 transfected 293T whole cell lysate. This data is not representative of endogenous protein expression, and the multiple bands observed may be the result of incomplete products of translation. The expected molecular weight for endogenous HSP 90 is ~ 90 kDa, and if multiple bands are observed this may also be due to post-translational modifications of the protein.
Answered by: Technical Support
Date published: 2020-03-11

Can this antibody be used for mouse Hsp90 protein in western blotting?

Asked by: Arundhati
Thank you for your question. This antibody has not been tested for mouse use, and can therefore not be warrantied for this use. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-05-24

This is Arun from india i am a research scholar and I am interested to buy this antibody before that I would like to test this antibody so how can i apply for free sample?

Asked by: Arun
Thank you for your question. We are not currently offering this specific antibody, HSP 90 (4F10): sc-69703, in a free sample size. As a possible alternative, we would recommend either our, HSP 90α (F-2): sc-515081, or our HSP 90α/β (F-8): sc-13119, both of which are available in a free sample size. Please contact one of our authorized distributors in India to request a sample. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-08-18

What is the recommended secondary reagent for detection of HSP 90 Antibody (4F10) via Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-20

Would it be possible for this antibody to work in avian samples?

Asked by: Trav11
To check additional species reactivity not listed on our website, please contact our Technical Service department at 800-457-3801 and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-20
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Rated 5 out of 5 by from WBできれいに検出できました抗体はWBに使用しました。サンプルはJurkatとMT-2細胞株。抗体の希釈倍率は1000倍。ブロッキング剤は市販のブロッキング剤または5%スキムミルクを使用しました。どちらのブロッキング剤を使用してもバックグラウンドはかなり低く、きれいなバンドが検出されました。
Date published: 2022-05-10
Rated 4 out of 5 by from HSP 90good antibody for Western blot, very clean for human cell extracts.
Date published: 2020-01-22
Rated 5 out of 5 by from GOODAntibody showed perfect bands in western blotting.
Date published: 2019-10-16
Rated 5 out of 5 by from Excellent antibody!!!Very strong bands in Western blot in 1:250 dilution, and also in 1:500 dilution, in mouse tumor tissues. Thank you santa cruz!
Date published: 2018-05-11
Rated 5 out of 5 by from Great antibody for western blotThe batch of antibody I have worked well on BJAB, HeLa, MCF7, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 200 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 4 out of 5 by from HSP 90 Antibody (4F10) ReviewMouse anti-HSP 90 Antibody (4F10) SAMPLE works verywell in WB at 1:1000 in 5 % BSA with LN229 cells and can be reused. And the bands were clear.
Date published: 2017-12-27
Rated 5 out of 5 by from Effect is goodUsed this antibody for several times, the effect were good. Mainly used in prostate cancer cells, 1:100 start, sample volume of 60 micrograms. High cost performance.
Date published: 2017-02-14
Rated 5 out of 5 by from strong and clearWestern blot strong and clear signal was got, by using HL-60, gingival fibroblast (hGF) cell and PBMC samples.
Date published: 2017-01-11
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HSP90 항체 (4F10) is rated 4.8 out of 5 by 19.
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