HKDC1 CRISPR/Cas9 KO Plasmid (m): sc-432046
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HKDC1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HKDC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HKDC1 CRISPR/Cas9 KO Plasmid (m) | sc-432046 | 20 µg | $397.00 |
Overview
Hkdc1 encodes HKDC1, a hexokinase domain-containing protein implicated in glucose phosphorylation and regulation of cellular glucose utilization. HKDC1 is linked to central carbon metabolism, contributing to glycolytic flux and coordination of energy homeostasis with mitochondrial function and nutrient sensing. In mouse systems, altered Hkdc1 activity has been associated with metabolic phenotypes involving glucose handling and insulin-responsive pathways, making it relevant to studies of metabolic dysregulation and stress responses in liver and other energy-demanding tissues. Its expression and functional coupling to carbohydrate metabolism support investigation into how hexokinase-like enzymes shape cellular adaptation to nutrient availability.
HKDC1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hkdc1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hkdc1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hkdc1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HKDC1 protein expression.
This CRISPR knockout system enables efficient generation of Hkdc1-deficient cell models for investigation of HKDC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hkdc1 exon(s) critical for HKDC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hkdc1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HKDC1 CRISPR/Cas9 KO Plasmid (m) and HKDC1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hkdc1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HKDC1 HDR Plasmid (m) and HKDC1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hkdc1 homology arms to support homology-directed repair at defined Hkdc1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.