Date published: 2025-9-7

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Histone H3 항체 (1G1): sc-517576

4.7(10)
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데이터 시트
  • Histone H3 항체 1G1 는 마우스 monoclonal IgG, 87간행물에 인용, 이며 100 µg/ml으로 제공합니다.
  • human 기원의 재조합 Histone H3 단백질 단편에 대해 항체 생성
  • WB와 IF으로 mouse, rat와 human origin의 Histone H3을 검출할것을 권장합니다.
  • Histone H3 (1G1): sc-517576무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP 는 Histone H3 항체 (1G1) for WB applications. 이 시약은 이제 Histone H3 항체 (1G1)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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히스톤 H3 항체(1G1)는 웨스턴 블롯팅(WB)과 면역형광(IF)을 통해 쥐, 쥐, 인간 샘플에서 히스톤 H3를 검출하는 쥐 단일 클론 IgG 항체입니다. 항-히스톤 H3 항체(1G1)는 비접합 형태로 제공됩니다. 히스톤 H3는 염색질 구조 조직화에 중요한 역할을 하며, 이는 핵 내 DNA 포장에 필수적입니다. 히스톤 H3는 DNA를 감싸는 뉴클레오좀의 8중 구조의 핵심 부분을 형성하여 유전자 발현과 DNA 복제를 조절합니다. 히스톤 H3는 게놈의 안정성을 유지하고 전사와 복구 과정을 촉진합니다. 히스톤 H3는 메틸화와 아세틸화를 포함한 다양한 번역 후 변형을 거치며, 이는 DNA와 단백질의 상호작용에 영향을 미치고, 염색질 구조와 기능에 영향을 미칩니다. 이러한 변형은 유전자 발현을 조절하고 세포 분화와 환경 반응에 영향을 미칩니다. 항-히스톤 H3 항체(1G1) 검출 기능은 다양한 생물학적 맥락에서 염색질 역학 및 후성유전학적 조절을 연구하는 데 유용한 시약입니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Histone H3 항체 (1G1)

sc-517576
100 µg/ml
$316.00

Histone H3 (1G1): m-IgG Fc BP-HRP 번들

sc-541115
100 µg Ab; 10 µg BP
$354.00

Histone H3 (1G1): m-IgG1 BP-HRP 번들

sc-542591
100 µg Ab; 20 µg BP
$354.00

I performed ICC with this antibody in mitotic cells, but I couldn't detect histone H3 signal co-localized with the DAPI signal. Could you help me solve this issue?

Asked by: KWJ94
Thank you for your product review. We are sorry to hear that this antibody did not perform for you as expected. There are many possible causes for no signal. Please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website, and we would be happy to help you figure this out. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase and will do our best to help you achieve the best results possible!
Answered by: Jiajun Z
Date published: 2024-09-29

Hi can we please get a sample of sc-517576? Do you have that available? We just purchased sc-390935 with Texas A&M University Biology Department, Dr. Deb Bell-Pedersen lab.

Asked by: Johnny
Thank you for contacting Santa Cruz Biotechnology. To request a sample please email scbt@scbt.com
Answered by: BlakeJ
Date published: 2024-06-18

Just received a free sample, which dilution would you recommend for nuclear extracts WB?

Asked by: IngOlmos
Thank you for your question. Dilution recommendations can be found in the product's datasheet, which is available right here on the product page. For western blots we recommend a starting dilution of 1:200, and a dilution range between 1:100-1:1000. When working with nuclear extracts, an amount of protein between 10–20 µg is appropriate.
Answered by: Tech Support Europe
Date published: 2023-05-15

could it be possible to buy directly bound to alexa488 or 594 please

Asked by: antonio blesa
Thank you for your question. If you are interested in this antibody directly conjugated with AF488 or AF594, please contact our our Technical Service. We should be able to provide this format as special order.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-31
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Rated 4 out of 5 by from Good work!!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2023-02-17
Rated 5 out of 5 by from H3 Ab for Western BlottingAsked for a sample before purchasing. Worked straight away on CRC cell lines HCT116 and DLD1 (drug treated and untreated) when loading 20 ug of whole protein lysates and using 1:500 dilution in 5% BSA/TBST. Would definitely recommend.
Date published: 2023-01-24
Rated 5 out of 5 by from western blotI used this antibody for my mouse primary neuron cells western blot. I got very nice and clean bolt!
Date published: 2022-08-24
Rated 5 out of 5 by from good and strongThe antibody was tested on whole lysate from liver cancer cell lines (JHH6 andHuh7) and immortalized hepatocytes (IHH). Antibody: sensitive and specific. Too strong signal with the dilution you recommend. Conditions need to be better set. Apparent MW: about 15kDa. Expected MW: 15kDa. • 40ug/lane cell lysate • Transfer 1h30 100V, PVDF (0.2 µm) • Blocking milk 4% in 0.1% Tween -PBS 1h RT • Anti-HISTONE H3 (1G1)(sc-517576) 1:200 O/N 4°C in blocking solution • Anti-mouse HRP 1:1000 1h RT in blocking solution • Developed with ECL Plus
Date published: 2022-06-09
Rated 5 out of 5 by from Very good!In MB231 cells, a clear band appeared after WB analysis 1: 2000 dilution.
Date published: 2022-03-18
Rated 4 out of 5 by from Useful for my purposesI asked it as free trial and i worked well enough, so I think I can use it for my purposes.
Date published: 2020-12-23
Rated 5 out of 5 by from Great for IFBought this antibody recently for the purpose of immunofluorescence on human neuroblastoma cell lines. It worked very well.
Date published: 2019-09-03
Rated 5 out of 5 by from Works very well with 1:5000 dilutionWorks very well with 1:5000 dilution, and I hope some more conjugation products can be developed with this antibody.
Date published: 2019-02-05
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Histone H3 항체 (1G1) is rated 4.7 out of 5 by 10.
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