Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Hhip CRISPR/Cas9 KO Plasmid (m): sc-420853
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hhip CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hhip genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hhip Antibody (5D11): sc-293265
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hhip CRISPR/Cas9 KO Plasmid (m) | sc-420853 | 20 µg | $397.00 |
Overview
Mouse Hhip (hedgehog-interacting protein) encodes a membrane-associated antagonist of Hedgehog signaling that binds Hedgehog ligands and limits pathway activation. By modulating ligand availability, Hhip contributes to control of developmental patterning, tissue homeostasis, and epithelial–mesenchymal interactions downstream of Sonic Hedgehog–GLI transcriptional programs. Altered Hhip expression or regulation has been linked to dysregulated Hedgehog pathway output implicated in congenital malformations and diverse pathophysiological states, including aberrant growth and fibrotic remodeling. As a pathway brake, Hhip is frequently studied in contexts where Hedgehog signaling influences cell fate decisions, proliferation, and differentiation.
Hhip CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hhip gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hhip together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hhip open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hhip protein expression.
This CRISPR knockout system enables efficient generation of Hhip-deficient cell models for investigation of Hhip signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hhip exon(s) critical for Hhip function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hhip genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hhip CRISPR/Cas9 KO Plasmid (m) and Hhip CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hhip locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hhip HDR Plasmid (m) and Hhip HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hhip homology arms to support homology-directed repair at defined Hhip target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.