Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GM-CSFRα CRISPR/Cas9 KO Plasmid (m2): sc-419841-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GM-CSFRα CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GM-CSFRα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GM-CSFRα Antibody (S-50): sc-456
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GM-CSFRα CRISPR/Cas9 KO Plasmid (m2) | sc-419841-KO-2 | 20 µg | $397.00 |
Overview
Csf2ra encodes the alpha chain of the granulocyte-macrophage colony-stimulating factor receptor (GM-CSFRα), which confers ligand-binding specificity and partners with the common beta chain to initiate signaling in myeloid cells. Upon GM-CSF engagement, this receptor complex activates JAK2/STAT5, MAPK/ERK, and PI3K/AKT pathways to regulate proliferation, survival, differentiation, and functional activation of granulocytes and macrophages. GM-CSFRα-dependent signaling influences inflammatory cytokine programs and innate immune cell homeostasis across hematopoietic tissues. Dysregulation of the GM-CSF axis is implicated in immune-mediated inflammation and myeloid dysfunction, making Csf2ra a relevant node for mechanistic studies in inflammatory and hematopoietic disease models.
GM-CSFRα CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Csf2ra gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Csf2ra together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Csf2ra open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GM-CSFRα protein expression.
This CRISPR knockout system enables efficient generation of Csf2ra-deficient cell models for investigation of GM-CSFRα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Csf2ra exon(s) critical for GM-CSFRα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Csf2ra genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GM-CSFRα CRISPR/Cas9 KO Plasmid (m) and GM-CSFRα CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Csf2ra locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GM-CSFRα HDR Plasmid (m) and GM-CSFRα HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Csf2ra homology arms to support homology-directed repair at defined Csf2ra target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.