Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GGT5 Double Nickase Plasmid (h): sc-405055-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GGT5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GGT5 Double Nickase Plasmid (h) and GGT5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GGT5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GGT5 Antibody (F-8): sc-373693
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GGT5 Double Nickase Plasmid (h) | sc-405055-NIC | 20 µg | $410.00 | |||
GGT5 Double Nickase Plasmid (h2) | sc-405055-NIC-2 | 20 µg | $410.00 |
Human GGT5 encodes gamma-glutamyltransferase 5, a cell-surface ectoenzyme that catalyzes gamma-glutamyl transfer reactions involved in glutathione and leukotriene metabolism. By processing gamma-glutamyl substrates, GGT5 contributes to extracellular glutathione turnover, amino acid salvage, and redox homeostasis, with downstream effects on oxidative stress signaling and inflammatory pathways. Its activity intersects with eicosanoid biology through leukotriene C4 conversion and can influence cellular responses in immune and epithelial contexts. Altered expression or activity of GGT5 has been investigated in relation to tissue inflammation, metabolic stress, and tumor-associated microenvironmental processes, making it relevant for mechanistic studies of redox and lipid mediator networks.
GGT5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GGT5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GGT5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GGT5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GGT5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.