GALR3 CRISPR/Cas9 KO Plasmid (h): sc-406303
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GALR3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GALR3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GALR3 CRISPR/Cas9 KO Plasmid (h) | sc-406303 | 20 µg | $397.00 |
Overview
GALR3 encodes galanin receptor 3, a class A GPCR that binds the neuropeptide galanin and related ligands to modulate neuronal excitability and neuroendocrine signaling. Upon activation, GALR3 primarily couples to inhibitory G proteins to influence adenylyl cyclase activity, ion channel regulation, and downstream MAPK-linked signaling networks, thereby shaping neurotransmitter release and stress-responsive circuits. Expression of GALR3 in the central nervous system and peripheral tissues connects it to pathways controlling mood, nociception, feeding behavior, and autonomic regulation. Altered galanin–receptor signaling, including changes in GALR3 expression or activity, has been explored in the context of neuropsychiatric and neurological phenotypes and in broader studies of GPCR-mediated signal transduction.
GALR3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GALR3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GALR3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GALR3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GALR3 protein expression.
This CRISPR knockout system enables efficient generation of GALR3-deficient cell models for investigation of GALR3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GALR3 exon(s) critical for GALR3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GALR3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GALR3 CRISPR/Cas9 KO Plasmid (h) and GALR3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GALR3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GALR3 HDR Plasmid (h) and GALR3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GALR3 homology arms to support homology-directed repair at defined GALR3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.