GALK2 Double Nickase Plasmid (h): sc-410788-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GALK2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GALK2 Double Nickase Plasmid (h) and GALK2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GALK2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GALK2 Double Nickase Plasmid (h) | sc-410788-NIC | 20 µg | $410.00 | |||
GALK2 Double Nickase Plasmid (h2) | sc-410788-NIC-2 | 20 µg | $410.00 |
GALK2 encodes galactokinase 2, a cytosolic carbohydrate kinase implicated in galactose and broader hexose metabolism through phosphorylation of galactose to galactose-1-phosphate. By influencing flux through the Leloir-related network and downstream nucleotide-sugar pools, GALK2 can affect glycosylation capacity and cellular energy balance. Altered handling of galactose intermediates and related metabolic stress responses has relevance to studies of inborn errors of metabolism and hepatocyte-like phenotypes in model systems. GALK2 is therefore commonly investigated in the context of metabolic pathway regulation, carbon utilization, and metabolic adaptation under nutrient perturbation.
GALK2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GALK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GALK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GALK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GALK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.