Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
G6PD Double Nickase Plasmid (h): sc-401019-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- G6PD Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- G6PD Double Nickase Plasmid (h) and G6PD Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting G6PD. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: G6PD Antibody (G-12): sc-373886
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
G6PD Double Nickase Plasmid (h) | sc-401019-NIC | 20 µg | $410.00 | |||
G6PD Double Nickase Plasmid (h2) | sc-401019-NIC-2 | 20 µg | $410.00 |
Human G6PD encodes glucose-6-phosphate dehydrogenase, the rate-limiting enzyme of the oxidative pentose phosphate pathway that generates NADPH and ribose-5-phosphate. NADPH supports reductive biosynthesis and maintains glutathione in a reduced state, linking G6PD activity to cellular redox homeostasis and protection from oxidative stress. G6PD function influences metabolic flux, nucleotide synthesis, and responses to reactive oxygen species in erythrocytes and many proliferative cell types. Genetic variation or reduced enzyme activity is associated with G6PD deficiency and can modulate susceptibility to oxidative damage and hemolytic stress in specific contexts, making G6PD a central target for studying redox biology and metabolism.
G6PD Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the G6PD locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within G6PD. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt G6PD function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of G6PD-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.