FSBA Antibody (FSBA 2F5/5): sc-53287

FSBA Antibody (FSBA 2F5/5) is a mouse monoclonal IgG antibody that detects FSBA protein of non-species reactive (NSR) origins by immunoprecipitation (IP) and immunohistochemistry. Anti-FSBA antibody (FSBA 2F5/5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FSBA protein plays a crucial role in cellular signaling pathways, particularly in kinase activity regulation, which is essential for numerous physiological processes such as cell growth, differentiation, and metabolism. By covalently modifying conserved lysines in nucleotide-binding sites of kinases, FSBA serves as an ATP-affinity reagent that helps identify ATP-binding sites, providing insights into kinase regulation mechanisms. This modification influences enzyme activity and interaction with various substrates, ultimately affecting cellular responses to external signals. FSBA′s selective inactivation of ATP-diphosphohydrolase in human placental mitochondria inhibits progesterone synthesis and oxygen consumption, highlighting implications in reproductive biology and energy metabolism. With versatile applications and critical role in kinase research, FSBA monoclonal antibody (FSBA 2F5/5) serves as an invaluable tool for scientists exploring cellular signaling complexities.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

FSBA Antibody (FSBA 2F5/5) References:

1. 5'-p-Fluorosulfonylbenzoyl adenosine inhibits progesterone synthesis in human placental mitochondria.  |  Flores-Herrera, O., et al. 2002. Biochim Biophys Acta. 1585: 11-8. PMID: 12457710
2. Identification of the ATP binding sites of the carbamyl phosphate synthetase domain of the Syrian hamster multifunctional protein CAD by affinity labeling with 5'-[p-(fluorosulfonyl)benzoyl]adenosine.  |  Kim, HS., et al. 1991. Biochemistry. 30: 10322-9. PMID: 1681900
3. Selective ion tracing and MSn analysis of peptide digests from FSBA-treated kinases for the analysis of protein ATP-binding sites.  |  Renzone, G., et al. 2006. J Proteome Res. 5: 2019-24. PMID: 16889425
4. Structure of xanthine dehydrogenase from chicken and rat liver: chemical modification of NAD binding site with 5'-FSBA.  |  Nishino, T., et al. 1989. Adv Exp Med Biol. 253B: 173-8. PMID: 2692433
5. Three copies of the beta subunit must be modified to achieve complete inactivation of the bovine mitochondrial F1-ATPase by 5'-p-fluorosulfonylbenzoyladenosine.  |  Bullough, DA. and Allison, WS. 1986. J Biol Chem. 261: 5722-30. PMID: 2871017
6. Identification of the catalytic subunit of an oligomeric casein kinase (G type). Affinity labeling of the nucleotide site using 5'-[p-(fluorosulfonyl)benzoyl]adenosine.  |  Feige, JJ., et al. 1983. Biochemistry. 22: 1452-9. PMID: 6573204
7. Antibodies to fluorylsulfonylbenzoyladenosine permit identification of protein kinases.  |  Parker, PJ. 1993. FEBS Lett. 334: 347-50. PMID: 8243645
8. FSBA modifies both alpha- and beta-subunits of F1 specifically and can be bound together with AXP at the same alpha-subunit.  |  Hartog, AF., et al. 1997. Biochim Biophys Acta. 1318: 107-22. PMID: 9030259
9. Inactivation of isocitrate dehydrogenase kinase/phosphatase by 5'-[p-(fluorosulfonyl)benzoyl]adenosine is not due to the labeling of the invariant lysine residue found in the protein kinase family.  |  Oudot, C., et al. 1998. Eur J Biochem. 258: 579-85. PMID: 9874226

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FSBA Antibody (FSBA 2F5/5) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FSBA Antibody (FSBA 2F5/5) Specifications

Alternate Product Name: FSBA

Product Clone Name: FSBA 2F5/5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: FSBA

Epitope Species: N/A

Product Reactivity/Detection: protein kinases labelled with FSBA

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IHC(P)

Vialing Information: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for FSBA Antibody (FSBA 2F5/5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin