FRS2 Antibody (A-5): sc-17841

FRS2 Antibody (A-5) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect the Fibroblast Growth Factor Receptor Substrate 2 (FRS2), also known as SNT1 or FGFR substrate 2. FRS2 monoclonal antibody (A-5) targets an epitope spanning amino acids 258-348 of human FRS2 and demonstrates robust reactivity with FRS2 from mouse, rat, and human sources, making anti-FRS2 antibody (A-5) versatile for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). FRS2 plays a pivotal role as a docking protein in the FGF and NGF signaling pathways, essential for the activation of the Ras/MAPK pathway by bridging receptor tyrosine kinases to key signaling molecules like GRB2 and SOS. Located primarily at the plasma membrane, the subcellular localization of FRS2 is crucial for its function in signal transduction, facilitating effective interactions with activated receptors. Structurally, FRS2 is characterized by four Grb2 binding sites, a myristylation sequence necessary for membrane anchoring, and a protein tyrosine phosphatase (PTP) domain, all of which are vital for its interactions and signaling capabilities. Post-translational modifications, including myristylation and tyrosine phosphorylation, are essential for FRS2′s membrane localization, stability, and its ability to recruit additional signaling proteins, thereby regulating the strength and duration of downstream signaling cascades. FRS2 interacts with several critical proteins such as GRB2, SOS, and components of the Ras/MAPK pathway, which are integral to processes like cell proliferation, differentiation, and survival. Understanding these interactions and modifications underscores FRS2′s significance in various cellular functions and its implications in diseases, including cancer. Available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® variants, FRS2 (A-5) antibody provides researchers with a versatile tool for exploring FRS2-mediated signaling pathways and their impact on cellular biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

FRS2 Antibody (A-5) References:

1. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.  |  Lowenstein, EJ., et al. 1992. Cell. 70: 431-42. PMID: 1322798
2. C. elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains.  |  Clark, SG., et al. 1992. Nature. 356: 340-4. PMID: 1372395
3. SNT, a differentiation-specific target of neurotrophic factor-induced tyrosine kinase activity in neurons and PC12 cells.  |  Rabin, SJ., et al. 1993. Mol Cell Biol. 13: 2203-13. PMID: 7681142
4. The function of GRB2 in linking the insulin receptor to Ras signaling pathways.  |  Skolnik, EY., et al. 1993. Science. 260: 1953-5. PMID: 8316835
5. Suc1-associated neurotrophic factor target (SNT) protein is a major FGF-stimulated tyrosine phosphorylated 90-kDa protein which binds to the SH2 domain of GRB2.  |  Ong, SH., et al. 1996. Biochem Biophys Res Commun. 225: 1021-6. PMID: 8780727
6. A lipid-anchored Grb2-binding protein that links FGF-receptor activation to the Ras/MAPK signaling pathway.  |  Kouhara, H., et al. 1997. Cell. 89: 693-702. PMID: 9182757
7. SHP2 associates directly with tyrosine phosphorylated p90 (SNT) protein in FGF-stimulated cells.  |  Ong, SH., et al. 1997. Biochem Biophys Res Commun. 238: 261-6. PMID: 9299490

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FRS2 Antibody (A-5) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-FRS2 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FRS2 Antibody (A-5) Specifications

Alternate Product Name: FRS2

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 258-348

Epitope Target: FRS2

Epitope Species: human

Product Reactivity/Detection: FRS2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for FRS2 Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin