FMNL1 Double Nickase Plasmid (h): sc-404817-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FMNL1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FMNL1 Double Nickase Plasmid (h) and FMNL1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FMNL1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FMNL1 Antibody (C-5): sc-390023
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FMNL1 Double Nickase Plasmid (h) | sc-404817-NIC | 20 µg | $410.00 | |||
FMNL1 Double Nickase Plasmid (h2) | sc-404817-NIC-2 | 20 µg | $410.00 |
Formin-like protein 1 (FMNL1) is an actin nucleation and elongation factor of the diaphanous-related formin family that promotes filament assembly and remodeling through its FH1/FH2 domains. It is highly expressed in hematopoietic lineages and supports cytoskeletal processes including cell polarization, adhesion, and directed migration, linking Rho-family GTPase signaling to dynamic actin organization. FMNL1 function contributes to immune cell trafficking and phagocytic or synapse-like interactions by coordinating protrusive structures and membrane-associated actin networks. Dysregulated FMNL1 expression or signaling has been associated with altered leukocyte behavior and has been reported in multiple hematologic and solid tumor contexts, making it relevant for mechanistic studies of invasion, immune regulation, and cytoskeleton-dependent phenotypes.
FMNL1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FMNL1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FMNL1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FMNL1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FMNL1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.