Date published: 2025-9-7

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ferritin heavy chain 항체 (B-12): sc-376594

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  • ferritin heavy chain 항체 B-12 는 마우스 monoclonal IgG2a κ ferritin heavy chain 항체, 79간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 ferritin heavy chain에서 내부에 위치한 131-183 아미노산을 항원으로 사용하였습니다.
  • ferritin heavy chain 항체 (B-12)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 ferritin heavy chain 를 감지하는 데에 추천한다.
  • 항-ferritin heavy chain 항체(B-12)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • ferritin heavy chain (B-12): sc-376594무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP는 ferritin heavy chain 항체 (B-12) WB 애플리케이션용입니다. 이 시약은 이제 ferritin heavy chain 항체 (B-12)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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페리틴 중쇄 항체(B-12)는 마우스, 쥐 및 인간 유래의 페리틴 중쇄 단백질을 WB, IP, IF 및 ELISA로 검출하는 IgG2a κ 마우스 단일 클론 페리틴 중쇄 항체(페리틴 중쇄 1 항체 또는 FHC 항체라고도 지정됨)입니다. 페리틴 중쇄 항체(B-12)는 비접합 항페리틴 중쇄 항체 형태와 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항페리틴 중쇄 항체 형태로 제공됩니다. 포유류의 페리틴은 페리틴 중쇄와 페리틴 경쇄라는 두 가지 유형의 폴리펩티드 사슬로 구성된 24개의 서브유닛으로 구성되어 있으며, 각각 고유한 기능을 가지고 있습니다. 페리틴 중쇄는 철 저장의 첫 단계인 FeII의 산화를 촉매하는 반면, 페리틴 경쇄는 페리히드라이트의 핵 형성을 촉진하여 FeIII의 저장을 가능하게 합니다. 맘마-리안 페리틴의 가장 두드러진 역할은 세포에 철 완충 능력을 제공하는 것입니다. 철 완충 외에도 중쇄 페리틴은 MCF-7 세포에서 티미딜레이트 합성의 제한 인자인 세포질 세린 하이드 록시 메틸 트랜스퍼 라제의 발현 증가를 통해 티미딘 생합성 조절에도 관여합니다. 경쇄 페리틴은 경쇄 페리틴이 과발현되는 유전성 고페리틴혈증 백내장 증후군과 노화 관련 백내장 발병의 중요한 요인인 산화 스트레스 등 최소 두 가지 메커니즘에 의해 백내장에 관여합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

ferritin heavy chain 항체 (B-12) 참고문헌:

  1. 수정체 내 높은 수준의 페리틴 경쇄 mRNA.  |  Cheng, Q., et al. 2000. Biochem Biophys Res Commun. 270: 349-55. PMID: 10753629
  2. 중쇄 페리틴은 세린 하이드 록시 메틸 트랜스퍼 라제 발현과 새로운 티미딘 생합성을 향상시킵니다.  |  Oppenheim, EW., et al. 2001. J Biol Chem. 276: 19855-61. PMID: 11278996
  3. 황화물은 포유류 페리틴의 효율적인 철 방출제입니다.  |  Cassanelli, S. and Moulis, J. 2001. Biochim Biophys Acta. 1547: 174-82. PMID: 11343803
  4. 아편 유도 케모카인 기능 장애의 새로운 원인인 신경 페리틴 중쇄의 조절.  |  Abt, AC. and Meucci, O. 2011. J Neuroimmune Pharmacol. 6: 466-76. PMID: 21465240
  5. 헴 옥시게나제-1에 의한 심근 페리틴 중쇄 결핍의 역작용.  |  Machado, SE., et al. 2022. Int J Mol Sci. 23: PMID: 35955444
  6. 페리틴 중쇄는 페록시레독신 6과 결합하여 세포 증식 및 이동을 억제합니다.  |  Di Sanzo, M., et al. 2022. Int J Mol Sci. 23: PMID: 36361777
  7. 핵 인자 Kappa B는 누클레오폴리헤드로바이러스 감염에 대한 누에의 반응으로 누에에서 페리틴 중쇄 발현을 촉진합니다.  |  Cui, Z., et al. 2023. Biol Trace Elem Res. 201: 3717-3728. PMID: 36418633
  8. 페리틴 중쇄는 거위 난포 과립구 세포의 3-니트로프로피온산 유발 산화 스트레스와 세포자멸을 개선하는 데 관여합니다.  |  Jiang, D., et al. 2023. Poult Sci. 102: 102606. PMID: 36940654
  9. 페리틴 중쇄 유전자 FTH1의 이형 접합성 넌센스 변이체가 신경 페리틴증을 유발합니다.  |  Shieh, JT., et al. 2023. HGG Adv. 4: 100236. PMID: 37660254
  10. 페리틴 중쇄는 조절 T 세포 계통의 안정성과 기능을 지원한다.  |  Wu, Q., et al. 2024. EMBO J. 43: 1445-1483. PMID: 38499786
  11. 인간 페리틴 유전자의 염색체 11번 및 19q13.3에 할당----19qter.  |  Worwood, M., et al. 1985. Hum Genet. 69: 371-4. PMID: 3857215
  12. 고해상도에서 재조합 인간 H와 말 L 페리틴의 3차원 구조를 비교합니다.  |  Hempstead, PD., et al. 1997. J Mol Biol. 268: 424-48. PMID: 9159481

주문정보

제품명카탈로그 번호 단위가격수량관심품목

ferritin heavy chain 항체 (B-12)

sc-376594
200 µg/ml
$316.00

ferritin heavy chain (B-12): m-IgGκ BP-HRP 번들

sc-523022
200 µg Ab, 40 µg BP
$354.00

ferritin heavy chain (B-12): m-IgG2a BP-HRP 번들

sc-546133
200 µg Ab; 10 µg BP
$354.00

ferritin heavy chain 항체 (B-12) AC

sc-376594 AC
500 µg/ml, 25% agarose
$416.00

ferritin heavy chain 항체 (B-12) HRP

sc-376594 HRP
200 µg/ml
$316.00

ferritin heavy chain 항체 (B-12) FITC

sc-376594 FITC
200 µg/ml
$330.00

ferritin heavy chain 항체 (B-12) PE

sc-376594 PE
200 µg/ml
$343.00

ferritin heavy chain 항체 (B-12) Alexa Fluor® 488

sc-376594 AF488
200 µg/ml
$357.00

ferritin heavy chain 항체 (B-12) Alexa Fluor® 546

sc-376594 AF546
200 µg/ml
$357.00

ferritin heavy chain 항체 (B-12) Alexa Fluor® 594

sc-376594 AF594
200 µg/ml
$357.00

ferritin heavy chain 항체 (B-12) Alexa Fluor® 647

sc-376594 AF647
200 µg/ml
$357.00

ferritin heavy chain 항체 (B-12) Alexa Fluor® 680

sc-376594 AF680
200 µg/ml
$357.00

ferritin heavy chain 항체 (B-12) Alexa Fluor® 790

sc-376594 AF790
200 µg/ml
$357.00

Hi, do you have any updates on the validation of these Ab (conjugated) for flow cytometry (FC)? Are there any publications where these Ab were used for FC?

Asked by: OA___
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-07-13

Has this antibody been tested for Flow cytometry? At SCBT or any other published literature?

Asked by: sbagla
Thank you for your question. The unconjugated antibody has not yet been tested in that application. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-10-15

Did you teste this antibody for detection of mouse Fth? Although it is recomended, I would like to know if it was actually tested.

Asked by: dlcosta83
Thank you for your question. Ferritin heavy chain Antibody (B-12): sc-376594 is recommended for detection of ferritin heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA and has been established for those species and applications in our labs. We base our recommendations on these tests. The antibody has also been used successfully with murine samples in a recent publication: PMID 30935115.
Answered by: Tech Support Europe
Date published: 2019-05-27

I wonder how was this antibody validated to not cross react with Ferritin light chain?, has it been tried to be plotted with ferritin composed out of only light chain (like Ferritin from spleen)? I would like to use it in sandwich ELISA

Asked by: Zaher
Thank you for your question. This monoclonal antibody is raised against an antigen that is specific to the ferritin heavy chain, and is not expected to cross-react with ferritin light chain. We have not tested this antibody in spleen.
Answered by: Technical Support
Date published: 2018-12-10

Hello, I would like to know if this antibody can be used to detect rat ferritin heavy chain? Thank you.

Asked by: Teddy Chen
Thank you for your question. We have not yet tested our monoclonal antibody, ferritin heavy chain (B-12): sc-376594, to confirm reactivity with rat samples. However, it is very likely that the antibody would react with rat ferritin heavy chain based on roughly 92% sequence identity shared between the immunogen and the published protein sequence for rat (accession: P19132). We are offering a free 10 µg sample of this antibody for a limited time. Please contact our Technical Service Department if you would like a sample to test the antibody in rat. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-03-02

What is the recommended secondary to use in Western Blot with this antibody?

Asked by: cjMara
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, Mouse IgG kappa BP HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-19

Does this antibody (B12) cross-react with the ferritin light chain ?

Asked by: sv24
Thank you for your question. We do not expect this antibody, ferritin heavy chain Antibody (B-12): sc-376594, to cross-react with the ferritin light chain. The immunogen used to develop the antibody does not share significant sequence identity with the ferritin light chain protein. However, the exact epitope recognized by this monoclonal antibody has not been determined.
Answered by: Technical Support
Date published: 2016-12-17
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Rated 5 out of 5 by from Strong bands!Very good antibody. Worked beautifully for westerns.
Date published: 2023-01-25
Rated 5 out of 5 by from Why there are two ferritin bands on your blotting?I got the same bands as your demo experiment, just curious why there are two bands for ferritin heavy chain
Date published: 2022-08-12
Rated 5 out of 5 by from Good stainingWorking perfect for IHC (1:100) in Sprague-Dawley rat spleen tissue (alcohol-fixed). No background.
Date published: 2022-07-14
Rated 5 out of 5 by from Good performance in WBWe are pleased to get clear band in WB with this antibody.
Date published: 2021-03-31
Rated 5 out of 5 by from WB, 1:200, KM12Working perfect for WB (1:200) in KM12 cell lysates (50 ug). No background, no unspecific bands.
Date published: 2017-10-11
Rated 4 out of 5 by from FTHThe FTH Antibody works very well for Western blot applications, showing strong and clear banding with minimal background noise present
Date published: 2017-07-31
Rated 4 out of 5 by from 有两条带这个抗体出现两个条带,我用的rat来源的细胞样品,一套在20kd左右,条带很浅,一条在35kd左右,条带深稀释倍数500。
Date published: 2017-07-17
Rated 5 out of 5 by from excellent for WBStrong, clear and right size band has been gotten by this specific antibody
Date published: 2017-04-11
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ferritin heavy chain 항체 (B-12) is rated 4.9 out of 5 by 14.
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