FAS Antibody (B-10): sc-8009

FAS Antibody (B-10) is a mouse monoclonal IgG1 kappa light chain antibody that specifically targets the FAS protein, also known as CD95, Fas cell surface death receptor, or TNFRSF6, in human samples. FAS mouse monoclonal antibody (B-10) can be utilized in various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). FAS protein plays a crucial role in regulating apoptosis, which is the process of programmed cell death that is essential for maintaining cellular homeostasis and immune system function. FAS protein mediates apoptosis to eliminate virus-infected or transformed cells, thereby contributing to the body′s defense mechanisms against malignancies and infections. FAS protein is a cell surface glycoprotein that belongs to a receptor family that includes CD40 and tumor necrosis factor receptors, and is expressed on a wide range of lymphoid cell lines. FAS protein interaction with FAS-L is vital for triggering the apoptotic signaling cascade, highlighting the importance of this pathway in immune responses. FAS monoclonal antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates, making this reagent a valuable tool for researchers studying apoptosis and immune regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

FAS Antibody (B-10) References:

1. Role of perforin in lymphocyte-mediated cytolysis.  |  Yagita, H., et al. 1992. Adv Immunol. 51: 215-42. PMID: 1502975
2. Aging correlates with decreased beta-cell proliferative capacity and enhanced sensitivity to apoptosis: a potential role for Fas and pancreatic duodenal homeobox-1.  |  Maedler, K., et al. 2006. Diabetes. 55: 2455-62. PMID: 16936193
3. Lack of UCP2 reduces Fas-mediated liver injury in ob/ob mice and reveals importance of cell-specific UCP2 expression.  |  Fülöp, P., et al. 2006. Hepatology. 44: 592-601. PMID: 16941708
4. A central role of perforin in cytolysis?  |  Podack, ER., et al. 1991. Annu Rev Immunol. 9: 129-57. PMID: 1910674
5. Role of Fas/Fas-L in vascular cell apoptosis.  |  Stoneman, VE. and Bennett, MR. 2009. J Cardiovasc Pharmacol. 53: 100-8. PMID: 19188840
6. Targeting Fas in osteoresorptive disorders.  |  Kovacic, N., et al. 2010. Expert Opin Ther Targets. 14: 1121-34. PMID: 20854180
7. Perforin-dependent and -independent pathways of cytotoxicity mediated by lymphocytes.  |  Young, JD., et al. 1988. Immunol Rev. 103: 161-202. PMID: 3292393
8. Lipid Nanoparticle Delivery of Fas Plasmid Restores Fas Expression to Suppress Melanoma Growth In Vivo.  |  Al Subeh, ZY., et al. 2022. ACS Nano. 16: 12695-12710. PMID: 35939651
9. Mechanism of lymphocyte-mediated cytotoxicity.  |  Henkart, PA. 1985. Annu Rev Immunol. 3: 31-58. PMID: 3904772
10. Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family.  |  Suda, T., et al. 1993. Cell. 75: 1169-78. PMID: 7505205
11. Fas and its ligand in a general mechanism of T-cell-mediated cytotoxicity.  |  Hanabuchi, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 4930-4. PMID: 7515183
12. The Fas protein is expressed at high levels on CD4+CD8+ thymocytes and activated mature lymphocytes in normal mice but not in the lupus-prone strain, MRL lpr/lpr.  |  Drappa, J., et al. 1993. Proc Natl Acad Sci U S A. 90: 10340-4. PMID: 7694292

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FAS Antibody (B-10) has 135 citations in a variety of scientific publications.

Primary antibodies like Anti-FAS Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FAS Antibody (B-10) Specifications

Alternate Product Name: CD95

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 316-335

Epitope Target: FAS

Epitope Species: human

Product Reactivity/Detection: FAS

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 135 citations in various scientific publications

Available Conjugates/Forms for FAS Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred FAS antibody is FAS Antibody (G-9): sc-74540.