FADS6 CRISPR/Cas9 KO Plasmid (m): sc-435879
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FADS6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FADS6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FADS6 CRISPR/Cas9 KO Plasmid (m) | sc-435879 | 20 µg | $397.00 |
Overview
Fatty acid desaturase 6 (FADS6) is a membrane-associated enzyme implicated in the introduction of double bonds into polyunsaturated fatty acids, influencing lipid composition and downstream lipid signaling. By shaping pools of bioactive fatty acids and derivatives, FADS6 can affect membrane fluidity, organelle function, and metabolic homeostasis in mouse cells and tissues. Fads6-related activity intersects with broader fatty acid desaturation and elongation networks that coordinate energy balance and inflammatory mediator availability. Dysregulation of desaturase-linked lipid pathways is frequently studied in the context of metabolic stress, hepatic lipid accumulation, neurodevelopmental lipid requirements, and inflammatory phenotypes in preclinical models.
FADS6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Fads6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Fads6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Fads6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FADS6 protein expression.
This CRISPR knockout system enables efficient generation of Fads6-deficient cell models for investigation of FADS6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Fads6 exon(s) critical for FADS6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Fads6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FADS6 CRISPR/Cas9 KO Plasmid (m) and FADS6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Fads6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FADS6 HDR Plasmid (m) and FADS6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Fads6 homology arms to support homology-directed repair at defined Fads6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.