Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FAAH Double Nickase Plasmid (h): sc-402470-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAAH Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FAAH Double Nickase Plasmid (h) and FAAH Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FAAH. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FAAH Antibody (27-Y): sc-100739
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAAH Double Nickase Plasmid (h) | sc-402470-NIC | 20 µg | $410.00 | |||
FAAH Double Nickase Plasmid (h2) | sc-402470-NIC-2 | 20 µg | $410.00 |
Fatty acid amide hydrolase (FAAH) is a membrane-associated serine hydrolase that terminates signaling by hydrolyzing endocannabinoids such as anandamide and related N-acylethanolamines. By controlling ligand availability for cannabinoid receptors and other lipid-sensing pathways, FAAH influences synaptic transmission, neuroinflammatory signaling, nociceptive processing, and metabolic homeostasis. FAAH activity is closely linked to lipid mediator turnover at the endoplasmic reticulum and intersects with broader arachidonic acid and eicosanoid networks. Dysregulated FAAH expression or activity has been studied in the context of pain biology, anxiety-related phenotypes, substance use behaviors, and inflammatory or metabolic disorders, making it a useful node for mechanistic studies of lipid signaling.
FAAH Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FAAH locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FAAH. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FAAH function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FAAH-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.