Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Evx-1 CRISPR/Cas9 KO Plasmid (m): sc-420249
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Evx-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Evx-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Evx-1 Antibody (397.3): sc-81962
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Evx-1 CRISPR/Cas9 KO Plasmid (m) | sc-420249 | 20 µg | $397.00 |
Overview
Evx1 encodes the homeobox transcription factor Evx-1, a developmental regulator that patterns the embryo and helps specify cell fate decisions along the anterior–posterior axis. In mouse, Evx-1 participates in transcriptional networks that coordinate morphogenesis, including gene programs linked to neural tube patterning, hindbrain/spinal cord regionalization, and somitic development. As a sequence-specific DNA-binding protein, Evx-1 influences downstream signaling and differentiation pathways by controlling lineage-restricted transcriptional states. Dysregulated homeobox gene activity, including altered EVX1 programs, is studied for its impact on congenital malformations and aberrant differentiation phenotypes in developmental models.
Evx-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Evx1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Evx1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Evx1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Evx-1 protein expression.
This CRISPR knockout system enables efficient generation of Evx1-deficient cell models for investigation of Evx-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Evx1 exon(s) critical for Evx-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Evx1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Evx-1 CRISPR/Cas9 KO Plasmid (m) and Evx-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Evx1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Evx-1 HDR Plasmid (m) and Evx-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Evx1 homology arms to support homology-directed repair at defined Evx1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.