Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ERM Double Nickase Plasmid (h): sc-401161-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ERM Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ERM Double Nickase Plasmid (h) and ERM Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ETV5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ERM Antibody (H-06): sc-100941
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERM Double Nickase Plasmid (h) | sc-401161-NIC | 20 µg | $410.00 | |||
ERM Double Nickase Plasmid (h2) | sc-401161-NIC-2 | 20 µg | $410.00 |
ETV5 encodes the ETS family transcription factor ERM, a nuclear DNA-binding protein that regulates gene expression programs involved in cell fate decisions, proliferation, and migration. ERM functions downstream of receptor tyrosine kinase signaling, including FGF/MAPK pathways, and integrates developmental and tissue remodeling cues by controlling transcription of growth and differentiation-associated targets. Dysregulated ETV5 activity has been linked to altered epithelial–mesenchymal dynamics, invasion-associated transcriptional states, and lineage plasticity in multiple disease contexts, making it a useful node for studying transcriptional network rewiring. As a sequence-specific regulator, ERM also serves as a tractable model for interrogating ETS-factor cooperativity, enhancer usage, and chromatin-dependent control of transcription.
ERM Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ETV5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ETV5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ETV5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ETV5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.