Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
emerin Double Nickase Plasmid (h): sc-401048-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- emerin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- emerin Double Nickase Plasmid (h) and emerin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EMD. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: emerin Antibody (H-12): sc-25284
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
emerin Double Nickase Plasmid (h) | sc-401048-NIC | 20 µg | $410.00 | |||
emerin Double Nickase Plasmid (h2) | sc-401048-NIC-2 | 20 µg | $410.00 |
EMD encodes emerin, an inner nuclear membrane protein that binds lamins and LEM-domain partners to organize the nuclear lamina and connect chromatin to the nuclear periphery. Emerin participates in nucleo-cytoskeletal coupling through the LINC complex, regulates mechanotransduction, and influences transcriptional programs involved in muscle differentiation and cell-cycle control. Disruption of emerin-dependent nuclear architecture is linked to altered genome stability, aberrant signaling, and changes in cellular mechanics. Variants in EMD are associated with X-linked Emery–Dreifuss muscular dystrophy and related laminopathy phenotypes, making it a useful locus for studying nuclear envelope biology and stress-responsive gene regulation.
emerin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EMD locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EMD. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EMD function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EMD-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.