ELFN1 Double Nickase Plasmid (m): sc-433965-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ELFN1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ELFN1 Double Nickase Plasmid (m) and ELFN1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Elfn1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ELFN1 Double Nickase Plasmid (m) | sc-433965-NIC | 20 µg | $410.00 |
Elfn1 encodes ELFN1, a postsynaptic leucine-rich repeat and fibronectin type III domain-containing adhesion protein that helps organize synaptic connectivity in the mouse nervous system. ELFN1 participates in trans-synaptic signaling by binding presynaptic partners such as metabotropic glutamate receptors, shaping neurotransmitter release probability and short-term synaptic plasticity. Through these interactions it contributes to circuit formation and excitation–inhibition balance, processes central to neuronal development and synaptic maturation. Altered ELFN1-associated signaling has been investigated in the context of neurodevelopmental and neuropsychiatric disease biology, where synapse specification and network homeostasis are frequently perturbed.
ELFN1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Elfn1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Elfn1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Elfn1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Elfn1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.