Date published: 2025-9-6

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E-cadherin 항체 (G-10): sc-8426

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데이터 시트
  • E-cadherin 항체 G-10 는 마우스 monoclonal IgG1 κ E-cadherin 항체, 629간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human의 E-cadherin의 성숙 체인 내에서 600-707 아미노산에 대해 제기됨
  • E-cadherin 항체 (G-10)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 E-cadherin 를 감지하는 데에 추천한다.
  • 항-E-cadherin 항체(G-10)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • E-cadherin (G-10): sc-8426무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP 는 E-cadherin 항체 (G-10) 가WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은E-cadherin 항체(G-10) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)。

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E-cadherin 항체(G-10)는 마우스, 쥐, 인간에서 유래된 E-cadherin 단백질을 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 면역조직화학, 효소결합 면역흡착 분석법(ELISA)을 통해 검출하는 마우스 단일클론 IgG1 kappa 경쇄 항체입니다. 항-E-카데린 항체(G-10)는 비접합 형태와 아가로스, 호스래디시 퍼옥시다아제(HRP), 피코에리트린(PE), 플루오레세인 이소티오시아네이트(FITC), 다중 알렉사 플루오르® 접합체를 포함한 다양한 접합 형태로 제공됩니다. CD324 또는 CDH1로도 알려진 E-cadherin은 세포 부착에 중요한 역할을 하는데, 이는 조직의 완전성을 유지하고 배아 발달 과정에서 형태 발생 과정을 촉진하는 데 필수적입니다. E-카데린 단백질은 주로 세포막에 위치하며, E-카데린은 인접한 세포와 접합부(adherens junction)를 형성함으로써 조직의 구조적 안정성에 기여합니다. 이러한 접합부에 E-카데린이 존재하는 것은 세포 신호 전달 경로의 조절과 상피 세포의 극성 유지에 필수적입니다. E-카데린 기능의 붕괴는 암 전이와 같은 다양한 병리학적 상태와 관련이 있으며, 세포 접착력의 상실은 세포 운동성과 침습성을 증가시킵니다. E-cadherin과 β-catenin과 같은 세포질 단백질과의 상호작용은 세포 통신과 신호 전달에서 E-cadherin의 중요성을 더욱 강조하여, E-cadherin 단일 클론 항체(G-10)를 이러한 중요한 생물학적 과정을 연구하는 데 유용한 도구로 만들어 줍니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

E-cadherin 항체 (G-10) 참고문헌:

  1. 고등급 전립선암에서는 세포 부착 분자 E-카데린의 발현이 감소하거나 존재하지 않습니다.  |  Umbas, R., et al. 1992. Cancer Res. 52: 5104-9. PMID: 1516067
  2. 마우스 P-카데린 유전자의 게놈 조직 및 염색체 매핑.  |  Hatta, M., et al. 1991. Nucleic Acids Res. 19: 4437-41. PMID: 1886768
  3. [유방암의 외과 적 치료].  |  Hirsch, HA. 1978. Gynakol Rundsch. 18: 132-41. PMID: 207622
  4. 카데린: 동물의 형태 형성을 조절하는 세포 간 접착 분자.  |  Takeichi, M. 1988. Development. 102: 639-55. PMID: 3048970
  5. 데스모솜 카데린: 성장하는 또 다른 다중 유전자 접착 분자군.  |  Koch, PJ. and Franke, WW. 1994. Curr Opin Cell Biol. 6: 682-7. PMID: 7833048
  6. 카데린과 카테닌: 배아 발달에서의 상호 작용과 기능.  |  Ranscht, B. 1994. Curr Opin Cell Biol. 6: 740-6. PMID: 7833053
  7. 5개의 인간 카데린을 복제하여 카데린 세포 외 도메인의 특징을 밝히고 구조적으로 다른 두 가지 유형의 카데린에 대한 추가 증거를 제공했습니다.  |  Tanihara, H., et al. 1994. Cell Adhes Commun. 2: 15-26. PMID: 7982033
  8. 인간 내피 세포의 세포-세포 접합부에서 카데린과 PECAM-1 사이의 공간적 및 시간적 관계.  |  Ayalon, O., et al. 1994. J Cell Biol. 126: 247-58. PMID: 8027182
  9. 카데린/카테닌 복합체 형성의 역학: 새로운 단백질 상호작용과 복합체 조립 경로.  |  Hinck, L., et al. 1994. J Cell Biol. 125: 1327-40. PMID: 8207061

주문정보

제품명카탈로그 번호 단위가격수량관심품목

E-cadherin 항체 (G-10)

sc-8426
200 µg/ml
$316.00

E-cadherin (G-10): m-IgG Fc BP-HRP 번들

sc-525454
200 µg Ab; 10 µg BP
$354.00

E-cadherin 항체 (G-10) AC

sc-8426 AC
500 µg/ml, 25% agarose
$416.00

E-cadherin 항체 (G-10) HRP

sc-8426 HRP
200 µg/ml
$316.00

E-cadherin 항체 (G-10) FITC

sc-8426 FITC
200 µg/ml
$330.00

E-cadherin 항체 (G-10) PE

sc-8426 PE
200 µg/ml
$343.00

E-cadherin 항체 (G-10) Alexa Fluor® 488

sc-8426 AF488
200 µg/ml
$357.00

E-cadherin 항체 (G-10) Alexa Fluor® 546

sc-8426 AF546
200 µg/ml
$357.00

E-cadherin 항체 (G-10) Alexa Fluor® 594

sc-8426 AF594
200 µg/ml
$357.00

E-cadherin 항체 (G-10) Alexa Fluor® 647

sc-8426 AF647
200 µg/ml
$357.00

E-cadherin 항체 (G-10) Alexa Fluor® 680

sc-8426 AF680
200 µg/ml
$357.00

E-cadherin 항체 (G-10) Alexa Fluor® 790

sc-8426 AF790
200 µg/ml
$357.00

Can I request estimate sheet ?

Asked by: Jkimlab
Thank you for your question. Please contact technical service scbt@scbt.com
Answered by: BlakeJ
Date published: 2023-07-11

can you recommend another positive tissue besides tonsil as control; please

Asked by: Lucytch
Thank you for your question. E-Cadherin is highly expressed in a number of tissues including endocrine tissues, lung, gastrointestinal tract, liver, and pancreas.
Answered by: Tech Support Europe
Date published: 2020-11-19

I'd like to use sc-8426 conjugated with Alexa Fluor488 for flow cytometry application. What isotype control antibody do you recommend to use?

Asked by: jet12
Thank you for your question. The appropriate isotype control for this antibody is normal mouse IgG1 Alexa Fluor® 488 sc-3890.
Answered by: Tech Support Europe
Date published: 2020-09-22

Hi, I used this product for IP experiments. Although I have several trials(e.g Protein G IP kit, agarose G magnetic beads and agorose G beads) I couldn't get any band for IP sample.It has H1419 lot no. Have you any recommendations(protocols, buffers etc)?

Asked by: Billgee
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody. We are happy to help! Here is a link to the recommended CoA. https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: Tech Service
Date published: 2019-12-06

Hi, ı want to learn if you have E-cadherin conjugated to agarose beads(for IP) and what is it cat no?Thank you so much.Bests

Asked by: Bilgeee
Thank you for your question. Yes, we offer this E-cadherin antibody conjugated to agarose. The catalog number is: sc-8426 AC.
Answered by: Tech Service
Date published: 2019-10-07

你好,我想申请免费的抗体,我想知道具体是怎么申请的

Asked by: vanny
感谢您的提问。关于试用装的申请,欢迎通过:电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For samples request, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-03-15

Hallo, Sie schrieben dieses Produkt wird in einer Menge von 200µg/ml verkauft, doch es handelt sich hier um die Konzentration. Welche Menge erhalte ich pro Verpackungseinheit?

Asked by: th_re
Thank you very much for your inquiry. This antibody will be provided as 200 µg IgG1 in 1 ml buffer. This is also the amount per packaging unit that you will receive.
Answered by: Technical Support Europe
Date published: 2017-02-10

What is the recommended secondary reagent to be used for Western blot with E-cadherin (G-10): sc-8426? 

Asked by: Dr Ninau Qelp
We recommend our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP). m-IgGκ BP-HRP: sc-516102 https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp
Answered by: Tech Service 2
Date published: 2016-12-16
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Rated 5 out of 5 by from good working antibodySpecific binding and clear fluorescence signal in differentiated respiratory epithelium from hNESPCs of human nasal polyps
Date published: 2025-08-19
Rated 2 out of 5 by from UnsureUnsure if the antibody works. A band was observed in cytoplasmic and nuclear extacr but not in total lysate, hence unsure if it works.
Date published: 2024-09-24
Rated 1 out of 5 by from Poor qualityHuge amount of non-specific binding, even with 3% BSA blocking for 1hr. This is supposed to be a cell-cell adhesion marker.
Date published: 2023-12-23
Rated 4 out of 5 by from Good antibodyI used anti-B-cadherin (1:200) and worked perfectly in my breast cancer cell lysates (WB)
Date published: 2023-11-21
Rated 1 out of 5 by from Does not work in IF as plasma membrane markerThe antibody was used for immunofluorescence with secondary anti-mouse antibody fused with Alexa488, in osteosarcoma cell lines U2OS and Saos2 and in WI-38 VA13 immortalized cell line. In our hands, the antibody could not be used as a marker of intercellular boundaries. In U2OS and WI-38 VA13 cell lines, the signal was diffused, presenting as a strong background from both nucleus and cytoplasm. In Saos2 cells, most of the diffuse signal was localized in the nucleus, with some signal appearing at plasma membrane—however, not enough to be used as a PM marker.
Date published: 2023-07-04
Rated 1 out of 5 by from Totally wrong, it does not work.I tried IF with this Antibody with different protocols using an epithelial mouse cell line and none of those worked, I did not get the common pattern of cells achieved with another commercial antibodies
Date published: 2022-03-21
Rated 1 out of 5 by from A disaster, it does not work as usualIF staining patterns are completely wrong, no cell-cell junctions stained, seems all cytoplasmic with just non-specific patterns. AVOID!
Date published: 2021-08-26
Rated 4 out of 5 by from Good productThe antibody works well for IHC staining performed on murine paraffin-embedded tissues. Specific and clear signal, no strong background detected. Highly recommended.
Date published: 2021-07-14
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E-cadherin 항체 (G-10) is rated 3.9 out of 5 by 74.
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