DZIP3 Double Nickase Plasmid (h): sc-403972-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DZIP3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DZIP3 Double Nickase Plasmid (h) and DZIP3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DZIP3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DZIP3 Antibody (C-1): sc-514725
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DZIP3 Double Nickase Plasmid (h) | sc-403972-NIC | 20 µg | $410.00 | |||
DZIP3 Double Nickase Plasmid (h2) | sc-403972-NIC-2 | 20 µg | $410.00 |
DZIP3 (DAZ interacting zinc finger protein 3) is a zinc finger–containing E3 ubiquitin ligase implicated in ubiquitin-dependent protein turnover and regulation of signaling networks that control cell growth and stress responses. Through its ubiquitination activity and protein–protein interactions, DZIP3 is linked to proteostasis, transcriptional regulation, and modulation of pathway dynamics that influence cellular homeostasis. Altered DZIP3 expression or activity has been reported in studies of tumor biology and other complex diseases, motivating mechanistic investigations into how DZIP3-dependent ubiquitination shapes downstream phenotypes. Functional interrogation of DZIP3 supports research into pathway crosstalk, context-specific signaling, and genotype–phenotype relationships in human cell systems.
DZIP3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DZIP3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DZIP3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DZIP3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DZIP3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.