DUSP18 Antibody (E-2): sc-376923

DUSP18 Antibody (E-2) is a mouse monoclonal IgM antibody that detects DUSP18 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DUSP18 (E-2) antibody is available as the non-conjugated form. DUSP18, also known as dual specificity protein phosphatase 18 or low molecular weight dual specificity phosphatase 20, is a critical enzyme composed of 188 amino acids that plays a vital role in cellular signaling by dephosphorylating both phosphothreonine and phosphotyrosine residues within mitogen-activated protein (MAP) kinases. This dual specificity is essential for the regulation of MAP kinase pathways, which are involved in various cellular processes such as growth, differentiation, and stress responses. The expression of DUSP18 is induced by various growth factors and cellular stresses, allowing DUSP18 to negatively regulate key MAP kinase superfamily members, including MAPK/ERK, SAPK/JNK, and p38, thereby maintaining cellular homeostasis. DUSP18 exhibits optimal enzymatic activity at a pH of 6.0 and a temperature of 55 degrees Celsius, with the highest expression levels found in the testis, brain, ovary, and liver. Notably, DUSP18 is inhibited by iodoarectic acid and activated by manganese ions, highlighting complex regulatory mechanisms. Additionally, DUSP18 demonstrates a preferential activity against phosphorylated tyrosine residues compared to threonine residues, further emphasizing specificity in cellular signaling pathways.

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DUSP18 Antibody (E-2) References:

1. A growing family of dual specificity phosphatases with low molecular masses.  |  Aoki, N., et al. 2001. J Biochem. 130: 133-40. PMID: 11432789
2. Identification and characterization of two novel low-molecular-weight dual specificity phosphatases.  |  Hood, KL., et al. 2002. Biochem Biophys Res Commun. 298: 545-51. PMID: 12408986
3. Molecular cloning and characterization of a novel dual-specificity phosphatase18 gene from human fetal brain.  |  Wu, Q., et al. 2003. Biochim Biophys Acta. 1625: 296-304. PMID: 12591617
4. Structure of human DSP18, a member of the dual-specificity protein tyrosine phosphatase family.  |  Jeong, DG., et al. 2006. Acta Crystallogr D Biol Crystallogr. 62: 582-8. PMID: 16699184
5. Dual specificity phosphotase 18, interacting with SAPK, dephosphorylates SAPK and inhibits SAPK/JNK signal pathway in vivo.  |  Wu, Q., et al. 2006. Front Biosci. 11: 2714-24. PMID: 16720344
6. Regulation of innate immunity by MAPK dual-specificity phosphatases: knockout models reveal new tricks of old genes.  |  Salojin, K. and Oravecz, T. 2007. J Leukoc Biol. 81: 860-9. PMID: 17289800
7. Targeting dual-specificity phosphatases: manipulating MAP kinase signalling and immune responses.  |  Jeffrey, KL., et al. 2007. Nat Rev Drug Discov. 6: 391-403. PMID: 17473844
8. Spatiotemporal regulation of ERK2 by dual specificity phosphatases.  |  Caunt, CJ., et al. 2008. J Biol Chem. 283: 26612-23. PMID: 18650424

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DUSP18 Antibody (E-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-DUSP18 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DUSP18 Antibody (E-2) Specifications

Alternate Product Name: DUSP18

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 1-29

Epitope Target: DUSP18

Epitope Species: human

Product Reactivity/Detection: DUSP18

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for DUSP18 Antibody (E-2): P: Blocking Peptide