Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DHODH CRISPR/Cas9 KO Plasmid (h): sc-402983
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DHODH CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DHODH genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DHODH Antibody (E-8): sc-166348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DHODH CRISPR/Cas9 KO Plasmid (h) | sc-402983 | 20 µg | $397.00 |
Overview
Human DHODH (dihydroorotate dehydrogenase) is a mitochondrial inner-membrane flavoprotein that catalyzes the rate-limiting oxidation of dihydroorotate to orotate in de novo pyrimidine biosynthesis, coupling nucleotide production to the respiratory chain via ubiquinone. By controlling UMP availability, DHODH supports DNA/RNA synthesis, cell-cycle progression, and metabolic adaptation during proliferative stress. Perturbation of DHODH activity reshapes nucleotide pools, replication dynamics, and mitochondrial redox balance, linking it to pathways governing genome maintenance and cellular bioenergetics. Dysregulated pyrimidine metabolism and DHODH dependency have been observed in multiple disease-relevant contexts, including cancer cell proliferation and immune-cell activation.
DHODH CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DHODH gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DHODH together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DHODH open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DHODH protein expression.
This CRISPR knockout system enables efficient generation of DHODH-deficient cell models for investigation of DHODH signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DHODH exon(s) critical for DHODH function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DHODH genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DHODH CRISPR/Cas9 KO Plasmid (h) and DHODH CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DHODH locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DHODH HDR Plasmid (h) and DHODH HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DHODH homology arms to support homology-directed repair at defined DHODH target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.