CYP27B1 Antibody (E-7): sc-518129
- CYP27B1 Antibody (E-7) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 264-286 of CYP27B1 of mouse origin
- recommended for detection of CYP27B1 of mouse and rat origin by WB, IP, IF and ELISA
- m-IgGκ BP-HRP is the preferred secondary detection reagent for CYP27B1 Antibody (E-7) for WB applications. This reagent is now offered in a bundle with CYP27B1 Antibody (E-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
CYP27B1 Antibody (E-7) is a mouse monoclonal IgM antibody that detects CYP27B1 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CYP27B1 is a crucial enzyme belonging to the cytochrome P450 family, specifically the XXVIIB subfamily, and consists of 508 amino acids. CYP27B1 is primarily located in the mitochondrion and is predominantly expressed in the kidney, where CYP27B1 plays a vital role in calcium metabolism by catalyzing the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D). This conversion is essential for maintaining normal bone growth and tissue differentiation, highlighting CYP27B1′s importance in overall skeletal health. Deficiencies or mutations in the CYP27B1 gene can lead to vitamin D-dependent rickets type 1 (VDDR-1), a serious autosomal recessive disorder characterized by early-onset rickets, hypocalcemia, and muscle weakness, underscoring the critical nature of CYP27B1 in human health. Anti-CYP27B1 antibody (E-7) is an invaluable tool for researchers studying vitamin D metabolism and related disorders.
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CYP27B1 Antibody (E-7) References:
- Enzymatic studies on the key enzymes of vitamin D metabolism; 1 alpha-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24). | Inouye, K. and Sakaki, T. 2001. Biotechnol Annu Rev. 7: 179-94. PMID: 11686044
- Decreased expression of CYP27B1 correlates with the increased aggressiveness of ovarian carcinomas. | Brożyna, AA., et al. 2015. Oncol Rep. 33: 599-606. PMID: 25501638
- Mechanistic homeostasis of vitamin D metabolism in the kidney through reciprocal modulation of Cyp27b1 and Cyp24a1 expression. | Meyer, MB. and Pike, JW. 2020. J Steroid Biochem Mol Biol. 196: 105500. PMID: 31629064
- Single Nucleotide Polymorphisms in 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase (CYP27B1) Gene: The Risk of Malignant Tumors and Other Chronic Diseases. | Latacz, M., et al. 2020. Nutrients. 12: PMID: 32197412
- CYP27B1 Gene Polymorphism rs10877012 in Patients Diagnosed with Colorectal Cancer. | Latacz, M., et al. 2020. Nutrients. 12: PMID: 32260235
- CYP27B1 Downregulation: A New Molecular Mechanism Regulating EZH2 in Ovarian Cancer Tumorigenicity. | Huo, X., et al. 2020. Front Cell Dev Biol. 8: 561804. PMID: 33163485
- Calcium-Sensing Receptors Control CYP27B1-Luciferase Expression: Transcriptional and Posttranscriptional Mechanisms. | Huang, A., et al. 2021. J Endocr Soc. 5: bvab057. PMID: 34337274
- The role of vitamin D-synthesizing enzyme CYP27B1 in systemic lupus erythematosus. | Luo, M., et al. 2022. Turk J Med Sci. 52: 984-989. PMID: 36326421
- Expression of Rat Cyp27b1 in HepG2 Cells Using Adenovirus Vector and Its Application to Evaluation of Self-Made and Commercially Available Anti-Cyp27b1 Antibodies. | Nagao, C., et al. 2023. J Nutr Sci Vitaminol (Tokyo). 69: 90-97. PMID: 37121728
- CYP27B1 Enzyme in Psoriasis: A Preliminary Study of Immunohistochemical Observations. | Paliu, IA., et al. 2023. Life (Basel). 14: PMID: 38276264
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP27B1 Antibody (E-7) | sc-518129 | 200 µg/ml | $322.00 | |||
CYP27B1 Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-525393 | 200 µg Ab, 40 µg BP | $361.00 |