COX11 CRISPR/Cas9 KO Plasmid (h): sc-405874
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COX11 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COX11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COX11 CRISPR/Cas9 KO Plasmid (h) | sc-405874 | 20 µg | $397.00 |
Overview
COX11 encodes a mitochondrial inner membrane protein required for cytochrome c oxidase (complex IV) biogenesis, functioning as a copper metallochaperone that helps deliver copper to the COX1 catalytic core. Through this role, COX11 supports oxidative phosphorylation, electron transport, and cellular ATP production, with downstream effects on mitochondrial membrane potential and redox homeostasis. Disruption of COX11-dependent assembly can impair respiratory capacity and promote secondary changes in reactive oxygen species signaling and metabolic stress responses. Altered complex IV function and mitochondrial proteostasis are frequently studied in the context of neuromuscular and neurodegenerative phenotypes, as well as cancer cell metabolic remodeling.
COX11 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COX11 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COX11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COX11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COX11 protein expression.
This CRISPR knockout system enables efficient generation of COX11-deficient cell models for investigation of COX11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COX11 exon(s) critical for COX11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COX11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COX11 CRISPR/Cas9 KO Plasmid (h) and COX11 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COX11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COX11 HDR Plasmid (h) and COX11 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COX11 homology arms to support homology-directed repair at defined COX11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.