COL1A2 항체 (H-9) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 Collagen α2 Type I을 검출할것을 권장합니다.

COL1A2 항체 (H-9): sc-376350

COL1A2 항체 (H-9) is rated 3.5 out of 5 by 6.
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데이터 시트

    • COL1A2 항체(H-9) 는 마우스 monoclonal IgG1κ, 9간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 Collagen α2 Type I에서 에 가까운 1021-1090 아미노산을 항원으로 사용하였습니다.
    • 안티-COL1A2 항체 (H-9)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 Collagen α2 Type I 를 감지하는 데에 추천한다.; 이외에, canine, bovine and porcine등 species와 반응할수 있습니다
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-COL1A2 항체 (H-9)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • COL1A2 (H-9): sc-376350무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

Are there any issues with using sc-376350: COL1A2 (H-9) mouse monoclonal primary antibody on mouse samples?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-376350 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 1 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:25
Date published: 2018-11-19
Rated 1 out of 5 by from 有很严重的问题 我们实验室之前用过的抗体Col1a2,可是我western blot实验中在70kd的位置只有很浅的条带,怎么办?
Date published: 2018-05-17
Rated 5 out of 5 by from It works for western blotting! It detected endogenous COL1A2 in immortalized HFFs (1:500 in 5% milk/TBS-Tween, overnight at 4C).
Date published: 2017-11-28
Rated 5 out of 5 by from Great for WB This antibody is good for COL1A2 in WB. Strong and clear band observed in WB of adipose tissue of mouse.
Date published: 2017-10-20
Rated 5 out of 5 by from Good choice for Western Blot This antibody shows good detection of COL1A2 at the high molecular weight with a variety of whole cell lysates.
Date published: 2017-02-07
Rated 4 out of 5 by from Worked well in Western blot applications Worked well in Western blot applications with CCD1064Sk. -SCBT QC
Date published: 2015-04-11
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