COL12A1 항체 (A-11) WB, IP, IF 와ELISA으로 human origin의 Collagen Type XII을 검출할것을 권장합니다.

COL12A1 항체 (A-11): sc-166020

COL12A1 항체 (A-11) is rated 3.7 out of 5 by 3.
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데이터 시트

    • 안티-COL12A1 항체 A-11 는 마우스 monoclonal IgG1 COL12A1 항체, 6간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against amino acids 1861-2140 mapping within an internal region of Collagen Type XII of human origin
    • COL12A1 항체 (A-11)는 WB, IP, IF and ELISA으로 human유래의 Collagen Type XII 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-COL12A1 항체 (A-11)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • COL12A1 (A-11): sc-166020무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

What is the recommended storage condition for COL12A1 (A-11): sc-166020?

Asked by: Randy McDonald
Thank you for your question. We recommend storing the antibody at 4° C.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 1 out of 5 by from Negative signal observed in IF in human skeletal Negative signal was observed in IF in human skeletal muscle. 1. Frozen skeletal muscle section was fixed with acetone in 10min. 2. Wash the section in PBS buffer for 3×5minutes. 3. Place setion in 3% Hydrogen peroxide /methanol for 10 minutes 4.Wash the section in PBS buffer for 3×5minutes. 5.Place setion in 10% normal goat serum for 20min 6.Incubate section with primary anitbody. Dilution:1:200 Diluent:1%BSA, 4℃,overnight 7.Wash the section in PBS buffer for 3×5minutes. 8.Incubate section with secondary anitbody. goat anti-mouse IgG ,ALEX FLOUR 555 Dilution:1:150 Diluent:10% goat serum,room temperature 40min. 9.Wash the section in PBS buffer for 3×5minutes. 10.Moutn the section and observe.
Date published: 2017-07-31
Rated 5 out of 5 by from Excellent! This antibody is great for western blot analysis of COL12A1 expression in CCD-1064Sk whole cell lysate.
Date published: 2017-02-10
Rated 5 out of 5 by from Antibody works in WB data using CCD Antibody works in WB data using CCD-1064Sk whole cell lysate.
Date published: 2015-04-01
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