CNBP CRISPR/Cas9 KO Plasmid (m): sc-419712
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CNBP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CNBP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CNBP Antibody (H-7): sc-515387
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNBP CRISPR/Cas9 KO Plasmid (m) | sc-419712 | 20 µg | $397.00 |
Overview
Cnbp encodes cellular nucleic acid binding protein (CNBP), a conserved zinc-finger protein that binds single-stranded DNA and RNA to regulate transcription and translation. CNBP participates in nucleic acid metabolism and control of gene expression programs linked to cell growth, stress responses, and immune-related signaling. It has been implicated in regulation of G-rich elements and modulation of pathways that influence cytokine expression and cellular differentiation. Altered CNBP function is associated with dysregulated gene expression states relevant to neuromuscular and inflammatory phenotypes, making it a useful node for mechanistic studies in mouse models.
CNBP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cnbp gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cnbp together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cnbp open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CNBP protein expression.
This CRISPR knockout system enables efficient generation of Cnbp-deficient cell models for investigation of CNBP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cnbp exon(s) critical for CNBP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cnbp genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CNBP CRISPR/Cas9 KO Plasmid (m) and CNBP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cnbp locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CNBP HDR Plasmid (m) and CNBP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cnbp homology arms to support homology-directed repair at defined Cnbp target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.