Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CMAS CRISPR/Cas9 KO Plasmid (h): sc-409575
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CMAS CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CMAS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CMAS Antibody (E-8): sc-398296
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CMAS CRISPR/Cas9 KO Plasmid (h) | sc-409575 | 20 µg | $397.00 |
Overview
CMAS encodes cytidine monophosphate N-acetylneuraminic acid synthetase, a nuclear enzyme that catalyzes conversion of Neu5Ac to CMP-Neu5Ac, the activated donor substrate required for sialylation reactions in the Golgi apparatus. By controlling CMP-sialic acid availability, CMAS influences glycoprotein and glycolipid sialylation, affecting cell–cell interactions, receptor signaling, immune recognition, and protein stability at the plasma membrane. CMAS activity links central carbohydrate metabolism to glycoconjugate biosynthesis within the sialic acid pathway and broader glycosylation networks. Altered sialylation patterns associated with dysregulated CMAS function are relevant to studies of congenital disorders of glycosylation, neurodevelopmental phenotypes, and cancer cell surface remodeling.
CMAS CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CMAS gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CMAS together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CMAS open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CMAS protein expression.
This CRISPR knockout system enables efficient generation of CMAS-deficient cell models for investigation of CMAS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CMAS exon(s) critical for CMAS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CMAS genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CMAS CRISPR/Cas9 KO Plasmid (h) and CMAS CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CMAS locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CMAS HDR Plasmid (h) and CMAS HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CMAS homology arms to support homology-directed repair at defined CMAS target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.