CLPTM1 Double Nickase Plasmid (m): sc-425178-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLPTM1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLPTM1 Double Nickase Plasmid (m) and CLPTM1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Clptm1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLPTM1 Antibody (G-7): sc-374619
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLPTM1 Double Nickase Plasmid (m) | sc-425178-NIC | 20 µg | $410.00 |
Clptm1 encodes CLPTM1, a multi-pass transmembrane protein predominantly localized to intracellular membranes, including the endoplasmic reticulum, where it is thought to contribute to membrane protein trafficking and cellular stress responses. In mouse and other mammalian systems, CLPTM1 has been linked to regulation of receptor and ion channel surface expression, implicating it in processes such as synaptic signaling and cellular adaptation to proteostatic challenge. Altered CLPTM1 expression has been associated with cancer-related phenotypes and drug response pathways in several model contexts, supporting investigation of its roles in survival signaling and cellular sensitivity to stressors. Functional studies often focus on how CLPTM1 influences membrane organization, transport, and downstream signaling networks relevant to neurological and oncogenic biology.
CLPTM1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Clptm1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Clptm1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Clptm1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Clptm1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.