Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Clathrin Light Chain A/CLTA Double Nickase Plasmid (h): sc-403962-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Clathrin Light Chain A/CLTA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Clathrin Light Chain A/CLTA Double Nickase Plasmid (h) and Clathrin Light Chain A/CLTA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLTA. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Clathrin Light Chain A/CLTA Double Nickase Plasmid (h) | sc-403962-NIC | 20 µg | $410.00 | |||
Clathrin Light Chain A/CLTA Double Nickase Plasmid (h2) | sc-403962-NIC-2 | 20 µg | $410.00 |
CLTA encodes clathrin light chain A, a core component of clathrin-coated vesicles that modulates clathrin lattice assembly and dynamics with clathrin heavy chain during endocytosis. CLTA supports receptor-mediated internalization, synaptic vesicle recycling, and trafficking between the plasma membrane, endosomes, and the trans-Golgi network, linking it to pathways controlling membrane composition and signaling attenuation. Through its role in cargo sorting and endosomal routing, CLTA influences turnover of receptors such as growth factor receptors and immune receptors, shaping downstream signaling and cellular homeostasis. Dysregulation of clathrin-mediated trafficking has been associated with neurodegenerative processes, altered metabolic signaling, and cancer-relevant changes in receptor recycling and nutrient uptake, making CLTA a useful node for mechanistic studies of vesicle biology.
Clathrin Light Chain A/CLTA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLTA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLTA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLTA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLTA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.