human origin의 C-C chemokine receptor gene 5 (CKR-5)에서 내부에 위치한 66-250 아미노산을 항원으로 사용하였습니다.
안티-CKR-5 항체 (D-6)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 CKR-5 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-CKR-5 항체 (D-6)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for CKR-5 Antibody (D-6) for WB applications. These reagents are now offered in bundles with CKR-5 Antibody (D-6) (see ordering information below).
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CKR-5 Antibody (D-6) is a high quality monoclonal CKR-5 antibody (also designated CCR5 antibody or CMKBR5 antibody) suitable for the detection of the CKR-5 protein of mouse, rat and human origin. CKR-5 Antibody (D-6) is available as both the non-conjugated anti-CKR-5 antibody form, as well as multiple conjugated forms of anti-CKR-5 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. C-C or β chemokine family members are characterized by a pair of adjacent cysteine residues and serve as potent chemoattractants and activators of monocytes and T cells. C-C chemokine receptor family members include CKR-1, CKR-2A, CKR-2B, CKR-3, CKR-4, CKR-5, CKR-6, CKR-7, CKR-8, CKR-9, CKR-10 and the Duffy blood group antigen. Each of these receptors are G protein-coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation. However, this receptor family has also been shown to facilitate viral infection. Termed a "co-receptor", CKR-5, along with CD4, has been shown to be a major receptor for HIV. CKR-5 tends to associate with macrophage-tropic viruses, such as macrophage tropic HIV-1, while CKR-2B and CKR-3 bind a minority of viruses.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What are the exact conditions used to obtain the Western blot data shown on your website for CKR-5 (D-6): sc-17833 monoclonal antibody?
Asked by: cjMara
Thank you for your question. We used this primary antibody at a dilution of 1:200 and incubated for one hour at room temperature in Blotto (sc-2333). The complete recommended protocol for Western blotting is provided below.