Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ChoK CRISPR/Cas9 KO Plasmid (h): sc-403793
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ChoK CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ChoK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ChoK Antibody (B-8): sc-376489
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ChoK CRISPR/Cas9 KO Plasmid (h) | sc-403793 | 20 µg | $397.00 |
Overview
CHKA encodes choline kinase alpha (ChoK), the rate-limiting enzyme that phosphorylates choline to phosphocholine in the Kennedy pathway for phosphatidylcholine biosynthesis. By controlling phosphocholine pools and membrane phospholipid production, ChoK links lipid metabolism to cell-cycle progression, membrane biogenesis, and signal transduction networks that depend on phosphatidylcholine-derived second messengers. Altered CHKA activity has been associated with metabolic reprogramming and dysregulated proliferation programs observed across multiple disease-relevant cellular contexts, making it a useful node for interrogating lipid-driven signaling. CHKA/ChoK studies commonly intersect with pathways governing membrane dynamics, stress responses, and oncogenic growth factor signaling.
ChoK CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CHKA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CHKA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CHKA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ChoK protein expression.
This CRISPR knockout system enables efficient generation of CHKA-deficient cell models for investigation of ChoK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CHKA exon(s) critical for ChoK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CHKA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ChoK CRISPR/Cas9 KO Plasmid (h) and ChoK CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CHKA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ChoK HDR Plasmid (h) and ChoK HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CHKA homology arms to support homology-directed repair at defined CHKA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.