Cdx1 CRISPR/Cas9 KO Plasmid (h): sc-402522
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdx1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cdx1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdx1 Antibody (D-4): sc-515146
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdx1 CRISPR/Cas9 KO Plasmid (h) | sc-402522 | 20 µg | $397.00 |
Overview
CDX1 encodes the caudal type homeobox transcription factor Cdx1, a sequence-specific DNA-binding regulator that helps pattern the anterior–posterior axis and supports intestinal epithelial differentiation. Cdx1 functions within homeobox-driven transcriptional programs and interfaces with pathways such as Wnt/β-catenin signaling that shape gut development, cell fate decisions, and regional identity. Altered CDX1 expression has been reported in gastrointestinal disease contexts, including colorectal tumor biology, where dysregulated differentiation and proliferative signaling are common features. As a lineage-associated transcription factor, CDX1 is frequently used to study mechanisms of epithelial maturation, metaplasia-like gene programs, and transcriptional network remodeling.
Cdx1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDX1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDX1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDX1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cdx1 protein expression.
This CRISPR knockout system enables efficient generation of CDX1-deficient cell models for investigation of Cdx1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDX1 exon(s) critical for Cdx1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDX1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cdx1 CRISPR/Cas9 KO Plasmid (h) and Cdx1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDX1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cdx1 HDR Plasmid (h) and Cdx1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDX1 homology arms to support homology-directed repair at defined CDX1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.