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CD68 항체 (3F103): sc-70761

4.5(13)
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데이터 시트
  • CD68 항체(3F103) 는 마우스 monoclonal IgG1κ, 50간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human 기원의 합성 펩타이드에 대해 제기됨
  • WB, IP, IF, IHC(P)와 FCM으로 mouse, rat와 human origin의 CD68을 검출할것을 권장합니다.
  • CD68 (3F103): sc-70761무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 CD68 항체 (3F103) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은CD68 항체(3F103) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

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CD68 항체(3F103)는 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 파라핀 포매 절편을 이용한 면역조직화학(IHCP), 유세포분석법(FCM)을 통해 쥐, 쥐, 인간에서 CD68을 검출하는 쥐 단일클론 IgG1 kappa 경쇄 항체입니다. 항-CD68 항체(3F103)는 비접합 형태로 이용 가능합니다. CD68은 마우스 항원 macrosialin과 동종이고, 램프-1과 램프-2를 포함하는 산성, 고도로 당화되는 리소좀 당단백질(LGP) 계열의 일원입니다. CD68은 면역 체계에서 중요한 역할을 담당하며, 주로 병원체 방어와 염증 조절에 필수적인 대식세포와 다른 골수성 세포의 표지자 역할을 합니다. CD68은 주로 간과 신장 세뇨관 및 사구체를 포함한 다양한 비조혈 조직의 세포질 과립과 세포질에 위치하며, 대식세포, 단핵구, 호중구, 호염기구, 대림프구의 표면에도 존재하지만, 그 양은 적습니다. 이러한 면역 세포에 CD68이 존재하는 것은 면역 반응과 조직 항상성에서 그 역할을 이해하는 데 도움이 되는 식별과 특성화에 필수적입니다. 또한, CD68과 같은 리소좀 당단백질은 리소좀 막의 주요 구성 요소로, 가수분해 효소에 의한 분해로부터 막을 보호하여 세포의 완전성과 기능을 유지하는 데 도움이 될 수 있습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

CD68 항체 (3F103)

sc-70761
200 µg/ml
$316.00

CD68 (3F103): m-IgG Fc BP-HRP 번들

sc-528652
200 µg Ab; 10 µg BP
$354.00

CD68 (3F103): m-IgGκ BP-HRP 번들

sc-521091
200 µg Ab, 40 µg BP
$354.00

CD68 (3F103): m-IgG1 BP-HRP 번들

sc-543054
200 µg Ab; 20 µg BP
$354.00

What concentration for Flow cytometry is proper to use? It is suggested in the datasheet to use 1 ug/million cells, however, what concentration of Ab is recommended?

Asked by: Anonymous
Thank you for your question. This antibody, CD68 antibody, sc-70761, is provided as 200µg in 1ml, so if you need to use 1µg you will need to take 5µl of antibody solution. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-11-19

Which is the volume of the vial? Because it is referenced as 200 µg/ml but recommended concentrations are not in µg/ml

Asked by: VíctorSrX
Thank you for your question. CD68 Antibody (3F103) is a mouse monoclonal IgG1 provided at 200 µg in 1 mL volume.
Answered by: Technical Support
Date published: 2017-02-09

What is the best secondary to use with this antibody in Western Blot?

Asked by: Anna99
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-17

Anyone knows which is the appropriate pH for Antigen Retrieval?

Asked by: VíctorSrX
Thank you for your question. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-01-11
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Rated 1 out of 5 by from Not good for WB; too faint even at 1:200THP1 macrophages and Raw macrophages showed very faint bands to none when loaded15- 80ug proteins/well for WB; used 1:200 at Room temp after repeated use of this antibody for 2 days in cold.
Date published: 2018-12-20
Rated 4 out of 5 by from Good ABThis antibody gave a good result in IHC on frozen human section in a dilution of 1:100.
Date published: 2018-04-03
Rated 5 out of 5 by from great for TAM detectingI am very glad to receive your prdoucts two weeks ago.we use this antibody to mark the monocytes cells isolated from the peripheral blood very well, which is very helpful to our research.
Date published: 2017-12-04
Rated 5 out of 5 by from CD 68 immunohistochemical staining for TAMsIt worked for our tumor tissue biopsy sample. I got excellent result in immunohistochemical staining by using this CD68 antibody for our tumor-associated macrophage study.
Date published: 2017-12-04
Rated 3 out of 5 by from for WB analysisThis antibody tried many times,at first no band.I tried different dilutions, different expose time,finally tried 1:200 and incubated 2hour at RT, however the band still not strong and the antibody conceration is a little low.
Date published: 2017-06-25
Rated 5 out of 5 by from Great CD68 antibodyCD68 (3F103) works great for WB and IF. Specific IF staining of methanol-fixed HeLa cells showing membrane localization.
Date published: 2017-01-11
Rated 5 out of 5 by from CD 68 immunohistochemical staining for SCLCIt worked for our FFPE format lymph node and lung tissue biopsy sample. We got excellent result in immunohistochemical staining by using this CD68 antibody for our macrophage phenotypic study.
Date published: 2016-12-13
Rated 5 out of 5 by from Highly recommendable antibody in IHC PHighly recommendable antibody in IHC P
Date published: 2015-12-07
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CD68 항체 (3F103) is rated 4.5 out of 5 by 13.
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