Date published: 2025-9-9

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CD45 항체 (MB4B4): sc-21739

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데이터 시트
  • CD45 항체 MB4B4 는 랫드 monoclonal IgG, 3간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • ARH-77(B-림프모세포계 세포주)에 대해 양성 반응이 나타난 mouse 기원
  • WB, IP, IF 와FCM으로 mouse origin의 CD45을 검출할것을 권장합니다.
  • IF, IHC(P) 와 FCM에 사용가능한 phycoerythrin or fluoroscein 결합제품이 있습니다.
  • See CD45 (35-Z6): sc-1178 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • 현재 CD45 항체(MB4B4)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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CD45는 조혈 세포들 사이에서 광범위하게 발현되는 막횡단 당단백질로 확인되었다. CD45의 다수의 동형들은 세포 유형에 따라 면역계 전체에 분포되어 있다. 이러한 동형들은 엑손 4, 5 및 6의 대체 스플라이싱 때문에 발생한다. 상응하는 단백질 도메인은 CD45RA(엑손 4), CD45RB(엑손 5), CD45RC(엑손 6) 및 CD45RO(엑손 4 내지 6)에 특이성이 있는 단일클론항체의 결합을 특징으로 한다. 이러한 동형들의 변이는 세포내 도메인이 보존되는 동안 CD45의 세포외 도메인에 국한된다. CD45는 TCR/CD3 복합체에 의한 효율적인 티로신 인산화 유도를 위한 필수 성분인 포스포티로신 포스파타제로서 기능한다. CD45의 티로신 포스파타제 활성은 보존된 세포내 도메인 내에 포함된다. Src 및 Syk 계열 단백질 티로신 키나제는 신호 캐스케이드를 개시하기 위해 TCR/CD3 복합체에 의해 활용된다. Lck, Fyn 및 ZAP-70을 포함한 이 두 계열의 여러 구성체는 CD45의 생리학적 기질과 관련이 있다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

CD45 항체 (MB4B4)

sc-21739
200 µg/ml
$316.00

CD45 항체 (MB4B4) APCC7

sc-21739 APCC7
100 tests in 2 ml
$369.00

CD45 항체 (MB4B4) FITC

sc-21739 FITC
200 µg/ml
$330.00

CD45 항체 (MB4B4) PE

sc-21739 PE
200 µg/ml
$343.00

Hello guys. Can we use CD45 (MB4B4) for flow cytometry detection in Human Clinical Samples? Does it come pre-conjugated with PE or APC or FITC or any other? If no conjugation is available how should we use the same in a clinical setting? Please reply asap

Asked by: Sumit Dey
Thank you for your question. Yes, this antibody is recommended for Flow Cytometry, however, we do not recommend nor warranty our products for clinical samples. This product is for research use only. We do not offer it conjugated with any of the conjugates, I would recommend another antibody against CD45 that does come conjugated: CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Alternatively, we can provide this mouse monoclonal antibody, CD45 conjugated with any of your desired conjugated, but this would be a special order. Please contact one of our Special Orders coordinators by email at specialorders@scbt.com for more details.
Answered by: Tech Service
Date published: 2017-03-31

Can CD45 (MB4B4): sc-21739 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (MB4B4): sc-21739 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2023-08-01
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Rated 5 out of 5 by from Strong band in WB in mouse cells at 1:500 dilutionStrong band in WB in mouse cells at 1:500 dilution.
Date published: 2015-09-23
Rated 4 out of 5 by from Used for IP with CD4 T cellsUsed for IP with CD4 T cells. -SCBT QC
Date published: 2015-05-17
Rated 4 out of 5 by from Clean band observed at correct MW in westernClean band observed at correct MW in western blot analysis of CD45 expression in BYDP whole cell lysate. -SCBT QC
Date published: 2015-04-18
Rated 4 out of 5 by from Successful pulldown of CD45 from murine TSuccessful pulldown of CD45 from murine T-cells and CD4 cells (also used to detect via immunoblot). -SCBT Publication Review
Date published: 2015-02-24
Rated 4 out of 5 by from Produced wonderful FCM data for C57BL/6 mouseProduced wonderful FCM data for C57BL/6 mouse splenocytes. -SCBT QC
Date published: 2013-05-19
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CD45 항체 (MB4B4) is rated 4.2 out of 5 by 5.
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