CD36 항체 (185-1G2) IF와 FCM으로 mouse, rat와 human origin의 CD36을 검출할것을 권장합니다.

CD36 항체 (185-1G2): sc-21772

CD36 항체 (185-1G2) is rated 4.0 out of 5 by 3.
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데이터 시트

    • CD36 항체(185-1G2) 는 마우스 monoclonal IgG2aκ, 3간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against human leucocytes
    • IF와 FCM으로 mouse, rat와 human origin의 CD36을 검출할것을 권장합니다.
    • IF, IHC(P) 와 FCM에 권장하 phycoerythrin, FITC, Alexa Fluor® 488 또는 Alexa Fluor® 647 로 표기된 항체 200 µg/ml를 제공합니다 .
    • IF, IHC(P) 와 FCM에 사용가능한 PerCP 결합제품 이 있습니다.
    • See CD36 (SMφ): sc-7309 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can CD36 (185-1G2): sc-21772 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD36 (185-1G2): sc-21772 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

However, sc-21772 is AF 488, but not FITC, also can use sc-2856 as isotype? thanks.

Asked by: shang
Thank you for your question. If you are using this primary antibody directly conjugated to AF488, sc-21772 AF488: CD36 Antibody (185-1G2) then it is a mouse monoclonal with isotype IgG2a (kappa light chain). It is not suitable to use sc-2856 as an isotype control for this antibody because it is conjugated to FITC and not AF488. Our recommended isotype control for use with this AF488 conjugated primary antibody, is sc-3891: normal mouse IgG2a Alexa Fluor® 488. The link to that support product page is below. https://www.scbt.com/scbt/product/normal-mouse-igg2a-alexa-fluor-488
Answered by: Technical Support
Date published: 2016-09-30
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Rated 4 out of 5 by from Published IHCP data with human ovarian tumor Published IHCP data with human ovarian tumor - SCBT Publication Review
Date published: 2015-05-03
Rated 4 out of 5 by from Produced great immunofluorescence membrane Produced great immunofluorescence membrane staining in methanol-fixed THP-1 cells. -SCBT QC
Date published: 2015-04-13
Rated 4 out of 5 by from Good FCM data of human peripheral blood leukocytes Good FCM data of human peripheral blood leukocytes. -SCBT QC
Date published: 2014-08-23
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