Calpain 1 Antibody (5B3): sc-32796

Calpain 1 Antibody (5B3) is a mouse monoclonal IgG2a antibody that detects Calpain 1 in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Calpain 1, also known as µ-calpain, is an intracellular calcium-dependent protease that plays a crucial role in various cellular processes by cleaving cytoskeletal and submembranous proteins. Anti-Calpain 1 antibody (5B3) recognizes a protein essential for mediating specific calcium-dependent functions, including cell fusion, mitosis, and meiosis, which are vital for normal cellular function and development. The structure of Calpain 1 is characterized by its heterodimeric composition, consisting of a small regulatory subunit and a large catalytic subunit, which allows for precise regulation of proteolytic activity. Calpain 1′s regulation is further influenced by calpastatin, which inhibits both activity and binding to membranes, highlighting the importance of post-translational modifications in controlling function. Notably, Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain, suggesting involvement in neuroinflammatory processes. Calpain 1 is critical in spermatogenesis and the acrosome reaction, underscoring significance in reproductive biology. Calpain 1 (5B3) antibody serves as an invaluable tool for researchers studying cellular dynamics and signaling pathways.

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Calpain 1 Antibody (5B3) References:

1. Calpain-1 resident in lipid raft/caveolin-1 membrane microdomains plays a protective role in endothelial cells.  |  Martines, A., et al. 2017. Biochimie. 133: 20-27. PMID: 27956030
2. Impaired cerebellar plasticity and eye-blink conditioning in calpain-1 knock-out mice.  |  Heysieattalab, S., et al. 2020. Neurobiol Learn Mem. 170: 106995. PMID: 30735788
3. Calpain-2 participates in the process of calpain-1 inactivation.  |  Shinkai-Ouchi, F., et al. 2020. Biosci Rep. 40: PMID: 33078830
4. Calpain-1 mediates vascular remodelling and fibrosis via HIF-1α in hypoxia-induced pulmonary hypertension.  |  Deng, H., et al. 2022. J Cell Mol Med. 26: 2819-2830. PMID: 35365973
5. Calpain-1: a Novel Antiviral Host Factor Identified in Porcine Small Intestinal Mucus.  |  Li, Y., et al. 2022. mBio. 13: e0035822. PMID: 36102516
6. Lipid Peroxidation Products Influence Calpain-1 Functionality In Vitro by Covalent Binding.  |  Zhai, C., et al. 2023. J Agric Food Chem. 71: 7836-7846. PMID: 37167568
7. Ginsenoside Rg1 attenuates diabetic vascular endothelial dysfunction by inhibiting the calpain-1/ROS/PKC-β axis.  |  Lu, M., et al. 2023. Life Sci. 329: 121972. PMID: 37482213
8. Calpain-3 not only proteolyzes calpain-1 and -2 but also is a substrate for calpain-1 and -2.  |  Ojima, K., et al. 2023. J Biochem. 174: 421-431. PMID: 37491733
9. Remote ischemic preconditioning reduces mitochondrial apoptosis mediated by calpain 1 activation in myocardial ischemia-reperfusion injury through calcium channel subunit Cacna2d3.  |  Liu, G., et al. 2024. Free Radic Biol Med. 212: 80-93. PMID: 38151212
10. TRPC6-Calpain-1 Axis Promotes Tubulointerstitial Inflammation by Inhibiting Mitophagy in Diabetic Kidney Disease.  |  Liu, CC., et al. 2024. Kidney Int Rep. 9: 3301-3317. PMID: 39534194

The preferred Calpain 1 antibody is Calpain 1 Antibody (D-11): sc-271313.