C3 Antibody (B-9): sc-28294
- C3 Antibody (B-9) is a mouse monoclonal IgG1 κ C3 antibody, cited in 60 publications, provided at 200 µg/ml
- raised against amino acids 541-840 of C3 of human origin
- C3 Antibody (B-9) is recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-C3 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for C3 Antibody (B-9) for WB and IHC(P) applications. These reagents are now offered in bundles with C3 Antibody (B-9) (see ordering information below).
C3 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 541-840 of the human Complement Component 3 (C3) protein. C3, also known as CPAMD1 or acylation-stimulating protein (ASP), is a central component of the complement system, playing a crucial role in innate immunity. Synthesized primarily in the liver, C3 is secreted into the bloodstream and exists in the extracellular space, where C3 circulates as an inactive precursor. Upon activation, C3 is cleaved into C3a and C3b fragments; C3a functions as an anaphylatoxin mediating inflammatory responses, while C3b binds to pathogen surfaces, promoting opsonization and phagocytosis by immune cells. The extracellular location of C3 is essential, as C3 allows for immediate interaction with invading pathogens and activation of the complement cascade on cell surfaces. Dysregulation of C3 activation can lead to autoimmune disorders and increased susceptibility to infections. C3 (B-9) antibody recognizes the C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain, and C3b α′ chain of mouse, rat, and human origin, and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). C3 (B-9) monoclonal antibody is a valuable tool for studying complement activation pathways and their implications in immune responses across multiple species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
C3 Antibody (B-9) References:
- Modulation of C3a activity: internalization of the human C3a receptor and its inhibition by C5a. | Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
- Ligand-induced phosphorylation of anaphylatoxin receptors C3aR and C5aR is mediated by 'G protein-coupled receptor kinases. | Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
- A role for the C3a anaphylatoxin receptor in the effector phase of asthma. | Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
- Human complement component C3: cDNA coding sequence and derived primary structure. | de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
- Mitogen-activated protein kinase activation requires two signal inputs from the human anaphylatoxin C5a receptor. | Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
- TNF-alpha-mediated expression of the receptor for anaphylatoxin C5a on neurons in experimental Listeria meningoencephalitis. | Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C3 Antibody (B-9) | sc-28294 | 200 µg/ml | $322.00 | |||
C3 Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-528410 | 200 µg Ab; 10 µg BP | $361.00 | |||
C3 Antibody (B-9): m-IgGκ BP-HRP Bundle | sc-520784 | 200 µg Ab, 40 µg BP | $361.00 | |||
C3 Antibody (B-9) AC | sc-28294 AC | 500 µg/ml, 25% agarose | $424.00 | |||
C3 Antibody (B-9) HRP | sc-28294 HRP | 200 µg/ml | $322.00 | |||
C3 Antibody (B-9) FITC | sc-28294 FITC | 200 µg/ml | $336.00 | |||
C3 Antibody (B-9) PE | sc-28294 PE | 200 µg/ml | $349.00 | |||
C3 Antibody (B-9) Alexa Fluor® 488 | sc-28294 AF488 | 200 µg/ml | $364.00 | |||
C3 Antibody (B-9) Alexa Fluor® 546 | sc-28294 AF546 | 200 µg/ml | $364.00 | |||
C3 Antibody (B-9) Alexa Fluor® 594 | sc-28294 AF594 | 200 µg/ml | $364.00 | |||
C3 Antibody (B-9) Alexa Fluor® 647 | sc-28294 AF647 | 200 µg/ml | $364.00 | |||
C3 Antibody (B-9) Alexa Fluor® 680 | sc-28294 AF680 | 200 µg/ml | $364.00 | |||
C3 Antibody (B-9) Alexa Fluor® 790 | sc-28294 AF790 | 200 µg/ml | $364.00 |