Date published: 2025-9-6
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C3 Antibody (B-9) - Western Blotting - Image 10912
C3 항체(B-9): sc-28294. 인간 재조합 C3b의 웨스턴 블롯 분석.
C3 항체(B-9): sc-28294. 인간 재조합 C3b의 웨스턴 블롯 분석.

C3 항체 (B-9): sc-28294

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데이터 시트
  • C3 항체 B-9 는 마우스 monoclonal IgG1 κ C3 항체, 60간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 C3 precursor에서 내부에 위치한 541-840 아미노산을 항원으로 사용하였습니다.
  • C3 항체 (B-9)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain 를 감지하는 데에 추천한다.
  • 항-C3 항체(B-9)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • C3 (B-9): sc-28294의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP 와 m-IgGκ BP-HRP 는C3 항체(B-9) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은C3 항체(B-9) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)

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C3, C3a / α, C3 α, C3aR, C3d, C4, C4 α, C4 β, C5b-9 와C5 β를 위한 Complement Pathway Protein 항체

C3 항체(B-9)는 인간 보체 성분 3(C3) 단백질의 541-840 아미노산에 대해 생성된 쥐 단일 클론 IgG1 카파 경쇄 항체입니다. CPAMD1 또는 아실화 자극 단백질(ASP)로도 알려진 C3는 보체 시스템의 중심 구성 요소이며, 선천성 면역에서 중요한 역할을 합니다. 주로 간에서 합성되는 C3는 혈류로 분비되어 세포 외 공간에 존재하며, 이 공간에서 C3는 비활성 전구체로 순환합니다. 활성화되면 C3는 C3a와 C3b 조각으로 분해됩니다. C3a는 염증 반응을 매개하는 아나필라톡신으로 기능하는 반면, C3b는 병원체 표면에 결합하여 면역 세포의 식세포 작용과 오손화를 촉진합니다. C3의 세포 외 위치는 필수적입니다. C3는 침입하는 병원체와 즉각적인 상호 작용을 가능하게 하고 세포 표면에서 보체 캐스케이드의 활성화를 가능하게 하기 때문입니다. C3 활성화의 조절 장애는 자가면역 질환과 감염에 대한 취약성을 증가시킬 수 있습니다. C3(B-9) 항체는 C3 전구체, C3a 아나필라톡신, C3 α 사슬, C3 β 사슬, C3b α′ 사슬을 인식하며, 마우스, 쥐, 인간에서 유래된 C3(B-9) 항체는 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 파라핀 포매 절편을 이용한 면역조직화학(IHCP), 효소결합면역흡착분석법(ELISA)에 사용하도록 권장됩니다. C3(B-9) 단일 클론 항체는 여러 종에 걸친 면역 반응에서 보체 활성화 경로와 그 의미를 연구하는 데 유용한 도구입니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

C3 항체 (B-9) 참고문헌:

  1. C3a 활성의 조절: 인간 C3a 수용체의 내재화 및 C5a에 의한 억제.  |  Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
  2. 아나필라톡신 수용체 C3aR 및 C5aR의 리간드 유도 인산화는'G 단백질 결합 수용체 키나아제'에 의해 매개됩니다.  |  Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
  3. 천식의 이펙터 단계에서 C3a 아나필라톡신 수용체의 역할.  |  Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
  4. 인간 보체 성분 C3: cDNA 코딩 서열 및 파생된 기본 구조.  |  de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
  5. 미토겐 활성화 단백질 키나아제 활성화에는 인간 아나필라톡신 C5a 수용체로부터 두 가지 신호 입력이 필요합니다.  |  Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
  6. 실험적 리스테리아 뇌수막염에서 신경세포의 아나필라톡신 C5a 수용체의 TNF-알파 매개 발현.  |  Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605

주문정보

제품명카탈로그 번호 단위가격수량관심품목

C3 항체 (B-9)

sc-28294
200 µg/ml
$316.00

C3 (B-9): m-IgG Fc BP-HRP 번들

sc-528410
200 µg Ab; 10 µg BP
$354.00

C3 (B-9): m-IgGκ BP-HRP 번들

sc-520784
200 µg Ab, 40 µg BP
$354.00

C3 항체 (B-9) AC

sc-28294 AC
500 µg/ml, 25% agarose
$416.00

C3 항체 (B-9) HRP

sc-28294 HRP
200 µg/ml
$316.00

C3 항체 (B-9) FITC

sc-28294 FITC
200 µg/ml
$330.00

C3 항체 (B-9) PE

sc-28294 PE
200 µg/ml
$343.00

C3 항체 (B-9) Alexa Fluor® 488

sc-28294 AF488
200 µg/ml
$357.00

C3 항체 (B-9) Alexa Fluor® 546

sc-28294 AF546
200 µg/ml
$357.00

C3 항체 (B-9) Alexa Fluor® 594

sc-28294 AF594
200 µg/ml
$357.00

C3 항체 (B-9) Alexa Fluor® 647

sc-28294 AF647
200 µg/ml
$357.00

C3 항체 (B-9) Alexa Fluor® 680

sc-28294 AF680
200 µg/ml
$357.00

C3 항체 (B-9) Alexa Fluor® 790

sc-28294 AF790
200 µg/ml
$357.00

Hello-Which antibody pairs would you recommend to develop a C3 sandwich ELISA

Asked by: Aaron Stoker
Thank you for your question. We have not validated our antibodies for sandwich ELISA.
Answered by: BlakeJ
Date published: 2024-09-20

hello,i see C3 antibody (B-9): sc-28294 is a mouse monoclonal antibody,how can it detect c3a anaphylatoxin and C3b α′ chain at the same time,if i want to use it in IHC,how can i distinguish it is C3b,C3a or c3?

Asked by: sophia
Thank you for your question. C3 Antibody (B-9) is recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. If you would like to detect the specific protein, please select the other antibody. Please contact our Technical Service Department if you have any other questions. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Sen Li
Date published: 2023-12-25

hello,can C3 Antibody (B-9): sc-28294 be used to dectect C3a anaphylatoxin in mouse.If so,did you have any such successful examples (western images) to shown to me ?

Asked by: sophia0314
Thank you for your question. Yes, C3 Antibody (B-9): sc-28294 is recommended for detection of C3a anaphylatoxin in mouse sample by WB, IP, IF, IHC(P) and ELISA. We don't have an in-house data for detection of C3a anaphylatoxin in mouse sample by WB at this moment, but please refer to the citations for this product.
Answered by: Technical Service SH
Date published: 2023-03-16

Can I use non-transfected HEK-293T as a negative control? In your legend of Near-infrared western blot analysis of C3 expression said 293T has C3 band. But same time in your "sc-28294 HRP. Direct western blot analysis" legend said 293T has no band.

Asked by: Woosung
Thank you for your question. Our Control 293T Lysate: sc-117752 is derived from non-transfected 293T cells and suitable for use as a negative control with our 293T Transfected Lysates. Our Control 293T Lysate goes through the same transfection process as our gene-specific transfected lysates, except without transfecting any cDNA into the cells. The Control 293T Lysate is also used at a lower concentration than regular cell lines for Western blotting. That said, it does appear that HEK293 does not have significant RNA expression of C3 per the Human Protein Atlas. We will review of the Near-infrared Western blot data showing a signal in HEK293T cell lysate, and if it can not be supported we will remove it.
Answered by: Technical Support
Date published: 2021-07-12

Has anyone used this AB for IHC in rat brain tissue? 

Asked by: Anonymous
Thank you for your question. We do not have publications specifically in rat brain tissue, but we have many publication in other rat tissues such as kidney, intesting and heart. We do have publications in mouse brain samples, so I would expect it to work perfectly fine in rat brain as well.
Answered by: Tech Service
Date published: 2021-06-01

Hi - I see that this antibody can detect C3 (which has a molecular weight of 150kDa), but that it is also recommended to detect C3a and C3b. How do I specifically probe for C3a and C3b separately on a western blot? Do I add in something to break C3 down?

Asked by: C3question
Thank you for your question. At this time, we are not able to provide any specific inductions or treatments for samples to increase expression of the different C3 subunits. This is a research-based question, and we would recommend you consult with the primary literature. There are many product citations using this antibody for Western blotting applications, so it may be useful to start by reviewing the citations on our website for this antibody.
Answered by: Technical Support
Date published: 2019-07-12

Has this antibody specificity been validated? If so, can you include the link to evidence/publication? Thank you.

Asked by: Shandiz
Thank you for your question. This antibody has many data images published on this product page, all showing validation. It also has many citations showing it’s use in publications. You can find them here: https://www.scbt.com/scbt/misc/citationResults.jsp ?_requestid=6883845
Answered by: Technical Support
Date published: 2019-05-29

Under what conditions have you demonstrated a positive C3 signal in human paraffin samples? I have tried numerous epitope retrieval protocols and seen nothing. I'm disappointed that you discontinued the goat polyclonal V-20 in favor of this product.

Asked by: squareshot
Thank you for your question. We are sorry to hear that this antibody is not performing for you as expected. We want you to be successful in your research! We use a 1:50 dilution with this antibody on paraffin sections, after a antigen retrieval in sodium citrate with heat. Please contact our Technical Service Department by phone, e-mail or live chat. We are more than happy to help you troubleshoot the antibody. Also, we do warranty our antibodies for one year from the date of purchase, so if we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
Answered by: Tech Service
Date published: 2017-04-12
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Rated 5 out of 5 by from Works well4% PFA fixed, frozen section, rat cochlea, IF microscopy. Worked well.
Date published: 2018-08-01
Rated 1 out of 5 by from Does not detect human C3 in paraffin tissuesI tried using this antibody to detect human C3 in paraffin-embedded tissues, using three different antigen retrieval techniques, and saw nothing. I also tried using the unconjugated antibody followed by an anti-mouse secondary, saw nothing. On the other hand, the discontinued goat polyclonal worked.
Date published: 2017-05-08
Rated 5 out of 5 by from Great for all manner of C3 detection.This is a proven winner in C3 detection. It has broad range or reactivity and great specificity. It will provide you the results and signal you want in all standard applications. It has been published with more than 10 times.
Date published: 2017-01-05
Rated 5 out of 5 by from Superb!!!Great western if and ihc results; strong signal and low background
Date published: 2017-01-05
Rated 4 out of 5 by from IHC-Psome macrophages were positive for C3 by this antobody in IHC-P method
Date published: 2016-12-30
Rated 5 out of 5 by from Great in multiple applicationsThis antibody works very well in Western Blot, IF and IHC(P)
Date published: 2016-12-23
Rated 5 out of 5 by from I used it to detect mouse complement C3bI used it to detect mouse complement C3b with western blot. I should say that it is a wonderful product which gives a clear and concise band without obvious mixed band. I do have bought many of your product (mainly antibodies), they all works well, and the price is acceptable. You helped me a lot in my experiments, and I will keep on using your product.
Date published: 2015-10-15
Rated 5 out of 5 by from Great stain in mouse intestinal tissuesGreat stain in mouse intestinal tissues.
Date published: 2015-08-06
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C3 항체 (B-9) is rated 4.7 out of 5 by 16.
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