C21orf62 CRISPR/Cas9 KO Plasmid (h): sc-406451
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C21orf62 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C21orf62 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C21orf62 CRISPR/Cas9 KO Plasmid (h) | sc-406451 | 20 µg | $397.00 |
Overview
C21orf62 (chromosome 21 open reading frame 62) encodes a poorly characterized human protein with emerging links to fundamental cell biology, including regulation of nuclear processes and cell-cycle–associated gene networks. Existing genomic and transcriptomic datasets suggest context-dependent expression and potential involvement in pathways that coordinate chromatin-associated events and cellular stress responses, although mechanistic details remain limited. Variation within the 21q genomic region has been explored in studies of neurodevelopmental and proliferative phenotypes, making C21orf62 relevant for investigating gene dosage effects and pathway perturbations. Loss-of-function interrogation can help define its contribution to transcriptional programs, cellular fitness, and phenotype modulation in disease-relevant model systems.
C21orf62 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the C21orf62 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C21orf62 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C21orf62 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C21orf62 protein expression.
This CRISPR knockout system enables efficient generation of C21orf62-deficient cell models for investigation of C21orf62 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C21orf62 exon(s) critical for C21orf62 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C21orf62 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C21orf62 CRISPR/Cas9 KO Plasmid (h) and C21orf62 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the C21orf62 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C21orf62 HDR Plasmid (h) and C21orf62 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by C21orf62 homology arms to support homology-directed repair at defined C21orf62 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.