C20orf111 Double Nickase Plasmid (h): sc-412210-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C20orf111 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C20orf111 Double Nickase Plasmid (h) and C20orf111 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OSER1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C20orf111 Double Nickase Plasmid (h) | sc-412210-NIC | 20 µg | $410.00 | |||
C20orf111 Double Nickase Plasmid (h2) | sc-412210-NIC-2 | 20 µg | $410.00 |
OSER1 (protein C20orf111) is a human endoplasmic reticulum–associated factor implicated in organizing ER membrane architecture and supporting secretory pathway homeostasis. Reported cellular roles for OSER1 include contributions to ER morphology, protein handling within the early secretory system, and coordination of stress-adaptive processes that interface with proteostasis networks. Altered ER structure and chronic ER stress signaling are broadly relevant to oncogenic remodeling and neurodegenerative mechanisms, making OSER1 a useful node for studying how membrane organization influences cell state. OSER1 expression and pathway context have been explored in relation to proliferation and stress-response phenotypes, supporting its inclusion in functional genomics studies of disease-associated cellular remodeling.
C20orf111 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OSER1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OSER1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OSER1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OSER1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.