C10 CRISPR/Cas9 KO Plasmid (m): sc-422846
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C10 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C10 CRISPR/Cas9 KO Plasmid (m) | sc-422846 | 20 µg | $397.00 |
Overview
Mouse Ccl6 encodes the chemokine C10, a secreted CC-family ligand that promotes chemotactic recruitment of monocytes/macrophages and other myeloid populations to sites of tissue stress. C10 engages chemokine receptor signaling to drive G protein–coupled pathways linked to actin remodeling, cell migration, and inflammatory cytokine networks, shaping leukocyte trafficking and innate immune activation. Ccl6 expression is commonly associated with macrophage-rich microenvironments and can influence processes such as wound repair, fibrotic remodeling, and host responses to infection. Dysregulated chemokine gradients involving C10 are relevant to experimental models of chronic inflammation where altered myeloid infiltration contributes to pathology.
C10 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ccl6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ccl6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ccl6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C10 protein expression.
This CRISPR knockout system enables efficient generation of Ccl6-deficient cell models for investigation of C10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ccl6 exon(s) critical for C10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ccl6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C10 CRISPR/Cas9 KO Plasmid (m) and C10 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ccl6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C10 HDR Plasmid (m) and C10 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ccl6 homology arms to support homology-directed repair at defined Ccl6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.